Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes are produced in Escherichia coli as His6-tagged protein fusions | Saccharolobus solfataricus |
wild-type and mutant enzymes are produced in Escherichia coli as His6-tagged protein fusions | Pyrococcus abyssi |
Protein Variants | Comment | Organism |
---|---|---|
A78R/N79H | mutation has no positive effect on activity at 80°C on Pyrococcus abyssi tRNAAsp(GUA) | Saccharolobus solfataricus |
I27K | mutation increases activity at 80°C on Pyrococcus abyssi tRNAAsp(GUA) 1.6fold | Saccharolobus solfataricus |
K19I | mutation decreases the activity at position 13 in the Pyrococcus abyssi tRNAAsp at 55°C. At 80°C the mutation has almost no negative effect on its activity towards the Pyrococcus abyssi tRNATyr(GUA) | Pyrococcus abyssi |
K19I/R78A/H79N | mutation completely abolishes the activity at position 13 in the Pyrococcus abyssi tRNAAsp at 55°C. At 80°C the mutation nearly abolishes activity towards the Pyrococcus abyssi tRNATyr(GUA) | Pyrococcus abyssi |
N79H | mutation has no positive effect on activity at 80°C on Pyrococcus abyssi tRNAAsp(GUA) | Saccharolobus solfataricus |
R78A/H79N | mutation decreases the activity at position 13 in the Pyrococcus abyssi tRNAAsp at 55°C. At 80°C the mutation has almost no negative effect on its activity towards the Pyrococcus abyssitRNATyr(GUA) | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Pyrococcus abyssi tRNATyr(GUA) uridine35 | Pyrococcus abyssi | residue 35 reinforces the stability of the anticodon stemloop by interaction with residue U33 (13). This structural stabilization may increase the aminoacylation efficiency of tRNATyr(GUA) by its cognate aminoacyl-tRNA synthetase | Pyrococcus abyssi tRNATyr(GUA) pseudouridine35 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
- |
- |
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes produced in Escherichia coli as His6-tagged protein fusions | Saccharolobus solfataricus |
wild-type and mutant enzymes produced in Escherichia coli as His6-tagged protein fusions | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Pyrococcus abyssi tRNAAsp(GUA) uridine13 | - |
Pyrococcus abyssi | Pyrococcus abyssi tRNAAsp(GUA) pseudouridine13 | - |
? | |
Pyrococcus abyssi tRNATyr(GUA) uridine13 | some activity at high temperature on tRNAs that contain a U residue at position 13. No activity of is detected at position 35 in the Sulfolobus solfataricus pre-tRNATyr(GUA), even at 80°C | Saccharolobus solfataricus | Pyrococcus abyssi tRNATyr(GUA) pseudouridine13 | - |
? | |
Pyrococcus abyssi tRNATyr(GUA) uridine13 | the enzyme modifies tRNAs at position 13 and at other positions as well as intron-less and intron-containing tRNAsTyr(GUA) at position 35 | Pyrococcus abyssi | Pyrococcus abyssi tRNATyr(GUA) pseudouridine13 | - |
? | |
Pyrococcus abyssi tRNATyr(GUA) uridine35 | residue 35 reinforces the stability of the anticodon stemloop by interaction with residue U33 (13). This structural stabilization may increase the aminoacylation efficiency of tRNATyr(GUA) by its cognate aminoacyl-tRNA synthetase | Pyrococcus abyssi | Pyrococcus abyssi tRNATyr(GUA) pseudouridine35 | - |
? | |
Pyrococcus abyssi tRNATyr(GUA) uridine35 | the enzyme modifies tRNAs at position 13 and at other positions as well as intron-less and intron-containing tRNAsTyr(GUA) at position 35 | Pyrococcus abyssi | Pyrococcus abyssi tRNATyr(GUA) pseudouridine35 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pab aPus7 | - |
Pyrococcus abyssi |
Sso aPus7 | - |
Saccharolobus solfataricus |