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Literature summary for 5.4.99.25 extracted from
- The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi 55 in both mitochondrial and cytoplasmatic tRNAs (1997), Nucleic Acids Res., 25, 4493-4499.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned in an Escherichia coli expression vector downstream of a His6-tag. The resulting recombinant protein Pus4 is expressed at high level | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNA uridine55 | Saccharomyces cerevisiae | Pus4 catalyses the formation of pseudouridine55 in both mitochondrial and cytoplasmic tRNAs | tRNA pseudouridine55 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P48567 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNA uridine55 | Pus4 catalyses the formation of pseudouridine55 in both mitochondrial and cytoplasmic tRNAs | Saccharomyces cerevisiae | tRNA pseudouridine55 | - |
? | |
| tRNA uridine55 | the purified Pus4p catalyzes the formation of pseudouridine55 in T7 in vitro transcripts of several yeast tRNA genes. In contrast to the known yeast pseudouridine synthase (Pus1) of broad specificity, no other uridines in tRNA molecules are modified by the cloned recombinant Pus4p | Saccharomyces cerevisiae | tRNA pseudouridine55 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pus4 | - |
Saccharomyces cerevisiae |
| tRNA:pseudouridine-55 synthase | - |
Saccharomyces cerevisiae |
| tRNA:PSI55 synthase | - |
Saccharomyces cerevisiae |
| YNL292w | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the disruption of the YNL292w gene in yeast, which has no significant effect on the growth of yeast cells, leads to the complete disappearance of the pseudouridine55 formation activity in a cell-free extract | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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