Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes D139T and D139N are overexpressed from plasmids | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D139N | mutation completely blocks pseudouridine formation in vivo and in vitro. The mutant rluD gene produces a protein capable of complete reversal of the growth defect (of Escherichia coli mutant with a truncation in the rluD gene) without concomitant pseudouridine formation | Escherichia coli |
D139T | mutation completely blocks pseudouridine formation in vivo and in vitro. The mutant rluD gene produces a protein capable of complete reversal of the growth defect (of Escherichia coli mutant with a truncation in the rluD gene) without concomitant pseudouridine formation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
23S rRNA uridine1911/uridine1915/uridine1917 | Escherichia coli | - |
23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P33643 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
23S rRNA uridine1911/uridine1915/uridine1917 | - |
Escherichia coli | 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RluD | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | a truncation mutant of the gene for RluD (responsible for synthesis of 23S rRNA pseudouridines 1911, 1915, and 1917) blocks pseudouridine formation and inhibits growth. RluD mutants D139T and D139N are completely inactive in vivo and in vitro. In vivo, the growth defect can be completely restored by transformation of an RluD-inactive strain with plasmids carrying genes for RluD D139T or RluD D139N. Pseudouridine sequencing of the 23S rRNA from these transformed strains demonstrates the lack of these pseudouridines. Pseudoreversion is not responsible because transformation with empty vector under identical conditions does not alter the growth rate | Escherichia coli |