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show all sequences of 5.4.99.20

The crystal structure of E. coli rRNA pseudouridine synthase RluE

Pan, H.; Ho, J.D.; Stroud, R.M.; Finer-Moore, J.; J. Mol. Biol. 367, 1459-1470 (2007)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
hanging drop vapour diffusion method, crystal structures of the C-terminal, catalytic domain of Escherichia coli RluE at 1.2 A resolution and of full-length RluE at 1.6 A resolution. The crystals of the full-length enzyme contain two molecules in the asymmetric unit and in both molecules the N-terminal domain is disordered. The higher resolution, truncated RluE structure is used for analysis and for comparison to other pseudouridine synthases
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24900
-
24900, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
23S rRNA uridine2457
Escherichia coli
pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome.The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome
23S rRNA pseudouridine2457
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P75966
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine2457
pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome.The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome
681422
Escherichia coli
23S rRNA pseudouridine2457
-
-
-
?
23S rRNA uridine2457
pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome
681422
Escherichia coli
23S rRNA pseudouridine2457
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
24900, SDS-PAGE
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapour diffusion method, crystal structures of the C-terminal, catalytic domain of Escherichia coli RluE at 1.2 A resolution and of full-length RluE at 1.6 A resolution. The crystals of the full-length enzyme contain two molecules in the asymmetric unit and in both molecules the N-terminal domain is disordered. The higher resolution, truncated RluE structure is used for analysis and for comparison to other pseudouridine synthases
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24900
-
24900, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
23S rRNA uridine2457
Escherichia coli
pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome.The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome
23S rRNA pseudouridine2457
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine2457
pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome.The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome
681422
Escherichia coli
23S rRNA pseudouridine2457
-
-
-
?
23S rRNA uridine2457
pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome
681422
Escherichia coli
23S rRNA pseudouridine2457
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
24900, SDS-PAGE
Escherichia coli
Other publictions for EC 5.4.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748400
Tillault
Molecular determinants for 23 ...
Escherichia coli
J. Mol. Biol.
430
1284-1294
2018
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1
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5
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1
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4
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1
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1
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8
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1
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1
1
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1
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5
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1
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4
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1
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8
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1
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1
1
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2
2
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729920
Kim
Transcriptional organization a ...
Streptococcus mutans, Streptococcus mutans UA159
J. Bacteriol.
194
1968-1978
2012
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15
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1
1
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681422
Pan
The crystal structure of E. co ...
Escherichia coli
J. Mol. Biol.
367
1459-1470
2007
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1
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1
1
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2
1
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706734
Del Campo
Identification and site of act ...
Escherichia coli
RNA
7
1603-1615
2001
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2
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1
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1
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1
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1
1
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