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Literature summary for 5.4.99.2 extracted from
- Characterization of methylmalonyl-CoA mutase involved in the propionate photoassimilation of Euglena gracilis Z (2010), Arch. Microbiol., 192, 437-446.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | MCM activity is inhibited completely by the addition of 3 mM Hg2+ | Euglena gracilis |  |
| additional information | Na+, K+, Ca2+, Fe2+, Mg2+, and Co2+ (all at 3 mM) and 3 mM EDTA do not cause inhibition of the MCM activity | Euglena gracilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0279 | - |
(R)-2-methylmalonyl-CoA | in 100 mM potassium phosphate buffer (pH 7.5), at 37°C | Euglena gracilis | |
| 0.3474 | - |
succinyl-CoA | in 100 mM potassium phosphate buffer (pH 7.5), at 37°C | Euglena gracilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Euglena gracilis | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
2 * 75000, SDS-PAGE | Euglena gracilis |
| 78250 | - |
2 * 78250, calculated from amino acid sequence | Euglena gracilis |
| 149000 | - |
gel filtration | Euglena gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | B7XBM0 | - |
- |
| Euglena gracilis Z | B7XBM0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, TSKgel phenyl-Toyopearl column chromatography, TSKgel QAE-Toyopearl column chromatography, Bio-Scale CHT2-1 column chromatography, UNO Q-1 column chromatography, and Superdex 200 gel filtration | Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.06 | - |
crude homogenate, at 40°C | Euglena gracilis |
| 21 | - |
after 350fold purification, at 40°C | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-methylmalonyl-CoA | - |
Euglena gracilis | succinyl-CoA | - |
r | |
| (R)-2-methylmalonyl-CoA | - |
Euglena gracilis Z | succinyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 75000, SDS-PAGE | Euglena gracilis |
| homodimer | 2 * 78250, calculated from amino acid sequence | Euglena gracilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCM | - |
Euglena gracilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Euglena gracilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | the enzyme, when incubated at various temperatures for 10 min at pH 7.0, is stable up to 25°C, and complete loss of activity is observed at 50°C | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Euglena gracilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5'-deoxyadenosylcobalamin | the apoenzyme is converted to a holoenzyme by incubation for 4 h at 4°C with 0.01 mM 5'-deoxyadenosylcobalamin, the purified enzyme contains one mole of prosthetic 5'-deoxyadenosylcobalamin per mole of subunit | Euglena gracilis | |
| adenosylcobalamin | the apparent Km value of the apoenzyme is 22.7 nM | Euglena gracilis | |
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Release 2023.1 (January 2023)
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