Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Propionibacterium freudenreichii subsp. shermanii |
Protein Variants | Comment | Organism |
---|---|---|
R207Q | active site residue, mutation lowering kcat 10000-fold and increasing KM for methylmalonyl-CoA over 30fold | Propionibacterium freudenreichii subsp. shermanii |
R207Q/Y86F | double mutant designed to mimic active site of isobutyryl-CoA mutase | Propionibacterium freudenreichii subsp. shermanii |
Y89F | active site residue, mutation lowering kcat and increasing KM for methylmalonyl-CoA | Propionibacterium freudenreichii subsp. shermanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isobutyryl-CoA | R207Q and Y89F/R207Q mutants show conversion of adenosylcobalamin to hydroxycobalamin, indicating inactivation of the enzyme | Propionibacterium freudenreichii subsp. shermanii | |
n-butyryl-CoA | R207Q and Y89F/R207Q mutants show conversion of adenosylcobalamin to hydroxycobalamin, indicating inactivation of the enzyme. No changes in wild-type and Y89F-mutant | Propionibacterium freudenreichii subsp. shermanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM not detectable, methylmalonyl-CoA, mutant Y89F/R207Q, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
additional information | - |
additional information | KM over 4, methylmalonyl-CoA, mutant R207Q, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
0.133 | - |
methylmalonyl-CoA | wild type, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
0.357 | - |
methylmalonyl-CoA | mutant Y89F, 37°C | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylmalonyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | succinyl-CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
MCM | - |
Propionibacterium freudenreichii subsp. shermanii |