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Literature summary for 5.4.99.2 extracted from

  • Vlasie, M.D.; Banerjee, R.
    When a spectator turns killer: suicidal electron transfer from cobalamin in methylmalonyl-CoA mutase (2004), Biochemistry, 43, 8410-8417.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Propionibacterium freudenreichii subsp. shermanii

Protein Variants

Protein Variants Comment Organism
R207Q active site residue, mutation lowering kcat 10000-fold and increasing KM for methylmalonyl-CoA over 30fold Propionibacterium freudenreichii subsp. shermanii
R207Q/Y86F double mutant designed to mimic active site of isobutyryl-CoA mutase Propionibacterium freudenreichii subsp. shermanii
Y89F active site residue, mutation lowering kcat and increasing KM for methylmalonyl-CoA Propionibacterium freudenreichii subsp. shermanii

Inhibitors

Inhibitors Comment Organism Structure
isobutyryl-CoA R207Q and Y89F/R207Q mutants show conversion of adenosylcobalamin to hydroxycobalamin, indicating inactivation of the enzyme Propionibacterium freudenreichii subsp. shermanii
n-butyryl-CoA R207Q and Y89F/R207Q mutants show conversion of adenosylcobalamin to hydroxycobalamin, indicating inactivation of the enzyme. No changes in wild-type and Y89F-mutant Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KM not detectable, methylmalonyl-CoA, mutant Y89F/R207Q, 37°C Propionibacterium freudenreichii subsp. shermanii
additional information
-
additional information KM over 4, methylmalonyl-CoA, mutant R207Q, 37°C Propionibacterium freudenreichii subsp. shermanii
0.133
-
methylmalonyl-CoA wild type, 37°C Propionibacterium freudenreichii subsp. shermanii
0.357
-
methylmalonyl-CoA mutant Y89F, 37°C Propionibacterium freudenreichii subsp. shermanii

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylmalonyl-CoA
-
Propionibacterium freudenreichii subsp. shermanii succinyl-CoA
-
r

Synonyms

Synonyms Comment Organism
MCM
-
Propionibacterium freudenreichii subsp. shermanii