Literature summary for 5.4.99.2 extracted from

Korotkova, N.; Lidstrom, M.E.
MeaB is a component of the methylmalonyl-CoA mutase complex required for protection of the enzyme from inactivation (2004), J. Biol. Chem., 279, 13652-13658.

Search for more literature for 5.4.99.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Methylorubrum extorquens
expression in Escherichia coli	Paraburkholderia fungorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64000	-	x * 78000, SDS-PAGE and deduced from nucleotide sequence of gene mcmA, x * 64000, SDS-PAGE and deduced from nucleotide sequence of gene mcmB	Methylorubrum extorquens
78000	-	x * 78000, SDS-PAGE and deduced from nucleotide sequence of gene mcmA, x * 64000, SDS-PAGE and deduced from nucleotide sequence of gene mcmB	Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-2-methylmalonyl-CoA	Thermobifida fusca	-	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Methylorubrum extorquens	-	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Halalkalibacterium halodurans	-	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Geobacter metallireducens	-	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Cupriavidus metallidurans	-	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Leptospira interrogans serovar lai str. 56601	-	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Propionibacterium freudenreichii subsp. shermanii	-	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Ralstonia solanacearum	-	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Methylorubrum extorquens	MCM forms a complex with MeaB	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	Paraburkholderia fungorum	MCM forms a complex with MeaB	succinyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus metallidurans	-	-	-
Geobacter metallireducens	-	-	-
Halalkalibacterium halodurans	-	-	-
Leptospira interrogans serovar lai str. 56601	Q8F222	-	-
Methylorubrum extorquens	-	-	-
Methylorubrum extorquens	Q6TMA2	beta subunit	-
Methylorubrum extorquens	Q84FZ1	alpha subunit	-
Paraburkholderia fungorum	-	-	-
Propionibacterium freudenreichii subsp. shermanii	P11652	small subunit	-
Propionibacterium freudenreichii subsp. shermanii	P11653	large subunit	-
Ralstonia solanacearum	Q8Y2U5	-	-
Thermobifida fusca	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methylorubrum extorquens
-	Paraburkholderia fungorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.4	-	-	Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-2-methylmalonyl-CoA	-	Thermobifida fusca	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	-	Methylorubrum extorquens	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	-	Halalkalibacterium halodurans	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	-	Geobacter metallireducens	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	-	Cupriavidus metallidurans	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	-	Leptospira interrogans serovar lai str. 56601	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	-	Paraburkholderia fungorum	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	-	Propionibacterium freudenreichii subsp. shermanii	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	-	Ralstonia solanacearum	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	MCM forms a complex with MeaB	Methylorubrum extorquens	succinyl-CoA	-	?
(R)-2-methylmalonyl-CoA	MCM forms a complex with MeaB	Paraburkholderia fungorum	succinyl-CoA	-	?

Subunits

Subunits	Comment	Organism
?	x * 78000, SDS-PAGE and deduced from nucleotide sequence of gene mcmA, x * 64000, SDS-PAGE and deduced from nucleotide sequence of gene mcmB	Methylorubrum extorquens

Synonyms

Synonyms	Comment	Organism
MCM	-	Thermobifida fusca
MCM	-	Halalkalibacterium halodurans
MCM	-	Geobacter metallireducens
MCM	-	Cupriavidus metallidurans
MCM	-	Leptospira interrogans serovar lai str. 56601
MCM	-	Propionibacterium freudenreichii subsp. shermanii
MCM	-	Methylorubrum extorquens
MCM	-	Paraburkholderia fungorum
MCM	-	Ralstonia solanacearum
mcmB	-	Methylorubrum extorquens
methylmalonyl-CoA mutase	-	Thermobifida fusca
methylmalonyl-CoA mutase	-	Halalkalibacterium halodurans
methylmalonyl-CoA mutase	-	Geobacter metallireducens
methylmalonyl-CoA mutase	-	Cupriavidus metallidurans
methylmalonyl-CoA mutase	-	Leptospira interrogans serovar lai str. 56601
methylmalonyl-CoA mutase	-	Propionibacterium freudenreichii subsp. shermanii
methylmalonyl-CoA mutase	-	Methylorubrum extorquens
methylmalonyl-CoA mutase	-	Paraburkholderia fungorum
methylmalonyl-CoA mutase	-	Ralstonia solanacearum

Cofactor

Cofactor	Comment	Organism	Structure
adenosylcobalamin	-	Thermobifida fusca
adenosylcobalamin	-	Methylorubrum extorquens
adenosylcobalamin	-	Halalkalibacterium halodurans
adenosylcobalamin	-	Geobacter metallireducens
adenosylcobalamin	-	Cupriavidus metallidurans
adenosylcobalamin	-	Leptospira interrogans serovar lai str. 56601
adenosylcobalamin	-	Propionibacterium freudenreichii subsp. shermanii
adenosylcobalamin	-	Paraburkholderia fungorum
adenosylcobalamin	-	Ralstonia solanacearum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)