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Literature summary for 5.4.99.19 extracted from
- Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli (1995), Biochemistry, 34, 8904-8913.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25836 | - |
x * 25836, calculated from sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 16S rRNA uridine516 | Escherichia coli | in vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins, suggesting that pseudouridylation occurs at an intermediate stage of 30S assembly in vivo | 16S rRNA pseudouridine516 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AA43 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 16S rRNA uridine516 | in vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins, suggesting that pseudouridylation occurs at an intermediate stage of 30S assembly in vivo | Escherichia coli | 16S rRNA pseudouridine516 | - |
? | |
| 16S rRNA uridine516 | the enzyme is specific for uridine516 in 16S rRNA. In vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5'-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins | Escherichia coli | 16S rRNA pseudouridine516 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25836, calculated from sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 16S PSI516 synthase | - |
Escherichia coli |
| 16S RNA pseudouridine 516 synthase | - |
Escherichia coli |
| 16S RNA PSI516 synthase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
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