Literature summary for 5.4.99.17 extracted from

Ideno, N.; Umeyama, S.; Watanabe, T.; Nakajima, M.; Sato, T.; Hoshino, T.
Alicyclobacillus acidocaldarius squalene-hopene cyclase the critical role of steric bulk at Ala306 and the first enzymatic synthesis of epoxydammarane from 2,3-oxidosqualene (2018), ChemBioChem, 19, 1873-1886 .

Search for more literature for 5.4.99.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Protein Variants

Protein Variants	Comment	Organism
A306F	the mutant produces apolypodatetraene, gamma-polypodatetraene and neopolypoda-5(6),13,17,21-teraene from squalene and polypoda-8(9),13,17,21-tetraen-3a-ol, polypoda-7(8),13,17,21-tetraen-3alpha-ol and polypoda-7(8),13,17,21-tetraen-3beta-ol from a racemic mixture of (3S)-2,3-oxidosqualene and (3R)-2,3-oxidosqualene	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
A306T	the mutant produces (20R)-dammara-13(17),24-dien-3a-ol, (20R)-dammara-13(17),24-dien-3b-ol, (20R)-dammara-12(13),24-dien-3a-ol, (20R)-dammara-12(13),24-dien-3b-ol and (20R,24R)-17-epi-epoxydammarane from a racemic mixture of (3S)-2,3-oxidosqualene and (3R)-2,3-oxidosqualene	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
A306V	the mutant produces apolypodatetraene, dammara-13(17)-24-diene, gamma-polypodatetraene, (17E)(13aH)-malabarica-14(27),17,21-triene, (20R)-dammara-12(13),24-diene, (20R)-eupha-7(8),24-diene, (20S)-tirucalla-7(8),24-diene, and 17-epi-dammara-20(21),24-diene from squalene	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
squalene	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	-	hop-22(29)-ene	-	?
squalene + H2O	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	-	hopan-22-ol	-	?

Organism

Organism	UniProt	Comment	Textmining
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	P33247	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-2,3-oxidosqualene + H2O	-	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	3alpha-hydroxyhopene + 3alpha-hydroxyhopanol	-	?
(3S)-2,3-oxidosqualene + H2O	-	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	3beta-hydroxyhopene + 3beta-hydroxyhopanol	-	?
squalene	-	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	hop-22(29)-ene	-	?
squalene + H2O	-	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	hopan-22-ol	-	?

Synonyms

Synonyms	Comment	Organism
SHC	-	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
squalene-hopene cyclase	-	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

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Release 2023.1 (January 2023)