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Literature summary for 5.4.99.17 extracted from

  • Eichhorn, E.; Locher, E.; Guillemer, S.; Wahler, D.; Fourage, L.; Schilling, B.
    Biocatalytic process for (-)-ambrox production using squalene hopene cyclase (2018), Adv. Synth. Catal., 360, 2339-2351 .
No PubMed abstract available
Search for more literature for 5.4.99.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Protein Variants

Protein Variants Comment Organism
F129L the mutant shows a (E,E)-homofarnesol conversion at 24 h of reaction which is comparable to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
F129L/F601Y the mutant shows lower (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
F129L/M132R/I432T the mutant shows much lower (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
F129L/M132R/I432T/F601Y the mutant shows much lower (E,E)-homofarnesol conversion compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
F601Y the mutant shows a (E,E)-homofarnesol conversion at 24 h of reaction which is comparable to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
M132R/A224V/I432T the mutant shows better (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
M132R/I432T the mutant shows better (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
M132R/I432T/F601Y the mutant shows much lower (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Q579H/F601Y the mutant shows better (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
T77A/I92V/F129L the mutant shows better (E,E)-homofarnesol conversion at 24 h of reaction compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
squalene Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
-
 hop-22(29)-ene
-
 ?
squalene + H2O Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
-
 hopan-22-ol
-
 ?

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 P33247
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-homofarnesol
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 (-)-ambrox + (3aS,5aS,9aS,9bS)-3a,6,6,9a-tetramethyldodeca-hydronaphtho[2,1-b]furan
-
 ?
(E,Z)-homofarnesol
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 (4Z,7aS,11aS)-5,8,8,11a-tetramethyl-2,3,6,7,7a,8,9,10,11,11a-decahydrobenzo[b]oxonine + 9-epi-ambrox
-
 ?
squalene
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 hop-22(29)-ene
-
 ?
squalene + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 hopan-22-ol
-
 ?

Synonyms

Synonyms Comment Organism
SHC
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
squalene hopene cyclase
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446