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Literature summary for 5.4.99.17 extracted from

  • Ceruti, M.; Balliano, G.; Rocco, F.; Lenhart, A.; Schulz, G.E.; Castelli, F.; Milla, P.
    Synthesis and biological activity of new iodoacetamide derivatives on mutants of squalene-hopene cyclase (2005), Lipids, 40, 729-735.
    View publication on PubMed
Search for more literature for 5.4.99.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM 105 Alicyclobacillus acidocaldarius

Protein Variants

Protein Variants Comment Organism
C25S/C50S/C435S/C455S/C537S quintuple mutant Alicyclobacillus acidocaldarius
C25S/C50S/D376C/C435S/C455S/C537S sextuple mutant Alicyclobacillus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
N-dodecyliodoacetamide IC50 wild-type >200 microMol, quintuple mutant >200 microMol, sextuple mutant >200 microMol, pH 6.0, 50°C Alicyclobacillus acidocaldarius
N-squalenyliodoacetamide IC50 wild-type >200 microMol, quintuple mutant >200 microMol, sextuple mutant 50 microMol, pH 6.0, 50°C Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information squalene sextuple mutant: no activity Alicyclobacillus acidocaldarius
0.001
-
 (3S)-2,3-oxidosqualene quintuple mutant, 50°C, pH 6.0 Alicyclobacillus acidocaldarius
0.0015
-
 (3S)-2,3-oxidosqualene wild-type, 50°C, pH 6.0 Alicyclobacillus acidocaldarius
0.0023
-
 (3S)-2,3-oxidosqualene sextuple mutant, 50°C, pH 6.0 Alicyclobacillus acidocaldarius
0.01
-
 squalene quintuple mutant, 50°C, pH 6.0 Alicyclobacillus acidocaldarius
0.0132
-
 squalene wild-type, 50°C, pH 6.0 Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-oxidosqualene
-
 Alicyclobacillus acidocaldarius lanosterol
-
 ?
squalene
-
 Alicyclobacillus acidocaldarius hopene
-
 ?

Synonyms

Synonyms Comment Organism
SHC
-
 Alicyclobacillus acidocaldarius

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.05
-
 IC50 sextuple mutant 50 microMol, pH 6.0, 50°C Alicyclobacillus acidocaldarius N-squalenyliodoacetamide
0.2
-
 IC50 wild-type >200 microMol, quintuple mutant >200 microMol, sextuple mutant >200 microMol, pH 6.0, 50°C Alicyclobacillus acidocaldarius N-dodecyliodoacetamide
0.2
-
 IC50 wild-type >200 microMol Alicyclobacillus acidocaldarius N-squalenyliodoacetamide
0.2
-
 IC50 quintuple mutant >200 microMol Alicyclobacillus acidocaldarius N-squalenyliodoacetamide