Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.16 extracted from

  • Lin, Y.F.; Su, P.C.; Chen, P.T.
    Production and characterization of a recombinant thermophilic trehalose synthase from Thermus antranikianii (2020), J. Biosci. Bioeng., 129, 418-422 .
    View publication on PubMed
Search for more literature for 5.4.99.16

Application

Application Comment Organism
synthesis good application potential of the recombinant enzyme in the efficient conversion of trehalose from maltose Thermus antranikianii

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed in Escherichia coli BL21(DE3) Thermus antranikianii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, 29% loss of activity Thermus antranikianii
Co2+ 1 mM, 56% loss of activity Thermus antranikianii
Cu2+ 1 mM, 83% loss of activity Thermus antranikianii
SDS 1 mM, 99% loss of activity Thermus antranikianii
Tris 1 mM, 46% loss of activity Thermus antranikianii
Zn2+ 1 mM, 33% loss of activity Thermus antranikianii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
73.2
-
 alpha,alpha-trehalose pH 7.0, 60°C Thermus antranikianii
112.43
-
 maltose pH 7.0, 60°C Thermus antranikianii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
112000
-
 SDS-PAGE Thermus antranikianii

Organism

Organism UniProt Comment Textmining
Thermus antranikianii WP_028494267
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus antranikianii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose 1.48fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose Thermus antranikianii maltose
-
 r
maltose 1.48fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose Thermus antranikianii alpha,alpha-trehalose
-
 r

Subunits

Subunits Comment Organism
? x * 112000, SDS-PAGE Thermus antranikianii

Synonyms

Synonyms Comment Organism
TaTS
-
 Thermus antranikianii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 highest activity (76.8%) Thermus antranikianii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 40% of maximal activity, 80°C: about 50% of maximal activity Thermus antranikianii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 6 h, 5% loss of activity Thermus antranikianii
80
-
 6 h, 50% loss of activity Thermus antranikianii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
64.84
-
 alpha,alpha-trehalose pH 7.0, 60°C Thermus antranikianii
148.04
-
 maltose pH 7.0, 60°C Thermus antranikianii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 highest activity and maximum trehalose yield Thermus antranikianii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 stable for 6 h Thermus antranikianii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.885
-
 alpha,alpha-trehalose pH 7.0, 60°C Thermus antranikianii
1.31
-
 maltose pH 7.0, 60°C Thermus antranikianii