Any feedback?
Literature summary for 5.4.99.16 extracted from
- The effect of high pressure on the intracellular trehalose synthase activity of Thermus aquaticus (2016), World J. Microbiol. Biotechnol., 32, 11 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | pressure as a stress condition can significantly increase the activity of intracellular enzyme TSase in Thermus aquaticus. After 2 h of culture under 1.0 MPa pressure, the activity of intracellular TSase in Thermus aquaticus reaches its maximum value. The bacteria are cultured at 70°C, 150 rpm, activity is highest at 1 MPa for 2 h | Thermus aquaticus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | Thermus aquaticus | - |
alpha,alpha-trehalose | - |
r |  |
| maltose | Thermus aquaticus SQ-11 | - |
alpha,alpha-trehalose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus aquaticus | - |
- |
- |
| Thermus aquaticus SQ-11 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ammonium sulfate fractionation, dialysis, and two steps of gel filtration, followed by ultrafiltration | Thermus aquaticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | analysis of enzyme activity at cell growth under pressure of 0.1-1.5 MPa and for 0.5-3 h, overview. For highest enzyme activity, the bacteria are cultured at 70°C, 150 rpm, at 1 MPa for 2 h, in a medium containing 30 g/l potassium glutamate | Thermus aquaticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
Thermus aquaticus | alpha,alpha-trehalose | - |
r | |
| maltose | - |
Thermus aquaticus SQ-11 | alpha,alpha-trehalose | - |
r | |
| additional information | improvement of activity by reaction condition optimization for trehalose production, overview. Pressure as a stress condition can significantly increase the activity of intracellular enzyme TSase in Thermus aquaticus | Thermus aquaticus | ? | - |
? | |
| additional information | improvement of activity by reaction condition optimization for trehalose production, overview. Pressure as a stress condition can significantly increase the activity of intracellular enzyme TSase in Thermus aquaticus | Thermus aquaticus SQ-11 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
Thermus aquaticus |
| TSase | - |
Thermus aquaticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermus aquaticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme has a strong thermal stability | Thermus aquaticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Thermus aquaticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
