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Literature summary for 5.4.99.16 extracted from
- A process for production of trehalose by recombinant trehalose synthase and its purification (2018), Enzyme Microb. Technol., 113, 83-90 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene treS, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | safe and reliable process of trehalose production by the recombinant enzyme expressed in Escherichis coli strain BL21(DE3). Vector pET15b-treS shows better plasmid stability and TreS expression, the highest activity, 39866 U/g dry cell weight occurs at a lactose concentration of 4 g/l for 7 h at 27°C in a 5-l fermentor at pH 8.0. The use of 30% w/v high-maltose syrup as a substrate can extend the temperature tolerance of enzyme TreS to 60°C. More than 64% of maltose can be converted into trehalose by adding 200 U of TreS per gram of maltose at 50°C for 24 h. The total sugar content of the trehalose syrup reached 95.0% w/w after separation. The recovery rate of trehalose dehydrate reaches 57.0% after slow cooling, and the purity is 99.0%. Method evaluation, detailed overview | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | Pseudomonas putida | - |
alpha,alpha-trehalose | - |
r |  |
| maltose | Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | - |
alpha,alpha-trehalose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q88FN0 | TreSB | - |
| Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | Q88FN0 | TreSB | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
Pseudomonas putida | alpha,alpha-trehalose | - |
r | |
| maltose | - |
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | alpha,alpha-trehalose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
Pseudomonas putida |
| TreS | - |
Pseudomonas putida |
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