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Literature summary for 5.4.99.16 extracted from
- Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli (2012), Acta Biochim. Pol., 59, 425-431.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| locus DR-2036, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of C-terminally His6-tagged enzyme in Escherichia coli strain (DE3) Rosetta pLysS from pET30Ek/LIC vector | Deinococcus radiodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 98.5% inhibition at 1 mM | Deinococcus radiodurans |  |
| Ca2+ | 13.4% inhibition at 10 mM, 9.5% at 1 mM | Deinococcus radiodurans | |
| Co2+ | 17.2% inhibition at 1 mM | Deinococcus radiodurans | |
| Cu2+ | complete inhibition at 10 mM | Deinococcus radiodurans | |
| dithiothreitol | 9% inhibition at 5 mM, 12% at 10 mM | Deinococcus radiodurans | |
| EDTA | 15% inhibition at 1 mM, 35% at 10 mM | Deinococcus radiodurans | |
| Hg2+ | complete inhibition at 1 mM | Deinococcus radiodurans | |
| Mg2+ | 8.5% inhibition at 1-10 mM | Deinococcus radiodurans | |
| Mn2+ | 20% inhibition at 1 mM, 30% at 10 mM | Deinococcus radiodurans | |
| Ni2+ | 28.5% inhibition at 1mM, 45% at 10 mM | Deinococcus radiodurans | |
| SDS | 95.1% inhibition at 5 mM | Deinococcus radiodurans | |
| Tris | 58% inhibition at 5 mM, 78% at 10 mM | Deinococcus radiodurans | |
| Zn2+ | complete inhibition at 5 mM | Deinococcus radiodurans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 290.7 | - |
maltose | pH 7.6, 30°C, recombinant His6-tagged enzyme | Deinococcus radiodurans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62710 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
| 64000 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
| 64690 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
| 126900 | - |
recombinant His6-tagged enzyme, gel filtration | Deinococcus radiodurans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | Deinococcus radiodurans | - |
alpha,alpha-trehalose | - |
? | |
| maltose | Deinococcus radiodurans DSM Z 20539 | - |
alpha,alpha-trehalose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | Q9RST7 | locus DR-2036 | - |
| Deinococcus radiodurans DSM Z 20539 | Q9RST7 | locus DR-2036 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged enzyme from Escherichia coli strain (DE3) Rosetta pLysS by cobalt affinity chromatography and ultrafiltration | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
Deinococcus radiodurans | alpha,alpha-trehalose | - |
? | |
| maltose | - |
Deinococcus radiodurans DSM Z 20539 | alpha,alpha-trehalose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
Deinococcus radiodurans |
| TreS | - |
Deinococcus radiodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Deinococcus radiodurans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 50 | activity range, profile, overview | Deinococcus radiodurans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
purified recombinant His6-tagged enzyme, 2 h, 45°C and pH 7.6, stable up to | Deinococcus radiodurans |
| 50 | - |
purified recombinant His6-tagged enzyme, 2 h, pH 7.6, inactivation | Deinococcus radiodurans |
| 50 | - |
purified recombinant His6-tagged enzyme, 8 h, pH 7.6, 56% activity remaining | Deinococcus radiodurans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Deinococcus radiodurans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | activity range, profile, overview | Deinococcus radiodurans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, inactivation | Deinococcus radiodurans |
| 5.5 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, 50% activity remaining | Deinococcus radiodurans |
| 7.6 | - |
purified recombinant His6-tagged enzyme, 2 h, 45°C and pH 7.6, stable | Deinococcus radiodurans |
| 9.5 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, 50% activity remaining | Deinococcus radiodurans |
| 10 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, inactivation | Deinococcus radiodurans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Deinococcus radiodurans | sequence calculation | - |
4.94 |
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