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Literature summary for 5.4.99.16 extracted from

  • Filipkowksi, P.; Panek, A.; Felcykowska, A.; Pieorow, O.; Synowiecki, J.
    Expression of Deinococcus geothermalis trehalose synthase gene in Escherichia coli and its einzymatic properties (2012), Afr. J. Biotechnol., 11, 13131-13139.
No PubMed abstract available
Search for more literature for 5.4.99.16

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM, 110% of initial activity Deinococcus geothermalis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Deinococcus geothermalis

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 55% residual activity Deinococcus geothermalis
Cu2+ 1 mM, 5% residual activity Deinococcus geothermalis
EDTA inhibitory above 1 mM Deinococcus geothermalis
Fe3+ 1 mM, 60% residual activity Deinococcus geothermalis
Hg2+ 1 mM, 15% residual activity Deinococcus geothermalis
Mg2+ 1 mM, 75% residual activity Deinococcus geothermalis
Tris 10 mM, 9% residual activity Deinococcus geothermalis
Zn2+ 1 mM, 29% residual activity Deinococcus geothermalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
254
-
 maltose pH 7.6, 40°C Deinococcus geothermalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6400
-
 x * 63620, calculated, x * 6400, SDS-PAGE of recombinant protein Deinococcus geothermalis
63620
-
 x * 63620, calculated, x * 6400, SDS-PAGE of recombinant protein Deinococcus geothermalis
132300
-
 gel filtration, recombinant protein Deinococcus geothermalis

Organism

Organism UniProt Comment Textmining
Deinococcus geothermalis Q1J0Z5 locus Dgeo-0537
-
Deinococcus geothermalis 11300 Q1J0Z5 locus Dgeo-0537
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.95
-
 pH 7.6, 40°C Deinococcus geothermalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose
-
 Deinococcus geothermalis alpha,alpha-trehalose
-
 ?
maltose
-
 Deinococcus geothermalis 11300 alpha,alpha-trehalose
-
 ?

Subunits

Subunits Comment Organism
dimer x * 63620, calculated, x * 6400, SDS-PAGE of recombinant protein Deinococcus geothermalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Deinococcus geothermalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 more than 80% of maximum activity Deinococcus geothermalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 half-life 42 h Deinococcus geothermalis
55
-
 8 h, 57% residual activity Deinococcus geothermalis
60
-
 2 h, 20% residual activity Deinococcus geothermalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.9
-
 maltose pH 7.6, 40°C Deinococcus geothermalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Deinococcus geothermalis

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.8 more than 80% of maximum activity Deinococcus geothermalis

pI Value

Organism Comment pI Value Maximum pI Value
Deinococcus geothermalis calculated
-
 4.9