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Literature summary for 5.4.99.16 extracted from

  • Yue, M.; Wu, X.L.; Gong, W.N.; Ding, H.B.
    Molecular cloning and expression of a novel trehalose synthase gene from Enterobacter hormaechei (2009), Microb. Cell Fact., 8, 34.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Enterobacter hormaechei

Inhibitors

Inhibitors Comment Organism Structure
beta-mercaptoethanol complete inhibition at 1 mM Enterobacter hormaechei
Cu2+ 52% residual activity at 1 mM Enterobacter hormaechei
Fe2+ 92% residual activity at 1 mM Enterobacter hormaechei
Hg2+ 57% residual activity at 1 mM Enterobacter hormaechei
additional information TreS activity is not affected by EDTA Enterobacter hormaechei
Na+ 90% residual activity at 1 mM Enterobacter hormaechei
SDS 10% residual activity at 1 mM Enterobacter hormaechei
Zn2+ 59% residual activity at 1 mM Enterobacter hormaechei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
25
-
maltose in 50 mM phosphate buffer (pH 6) at 37°C Enterobacter hormaechei
75
-
alpha,alpha-trehalose in 50 mM phosphate buffer (pH 6) at 37°C Enterobacter hormaechei

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ Mn2+ shows an enhancing effect by 11% at 1 mM Enterobacter hormaechei
additional information TreS activity is not affected by NH4+, Ca2+, and Mg2+ Enterobacter hormaechei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61800
-
predicted from amino acid sequence Enterobacter hormaechei
65000
-
His-tagged enzyme, SDS-PAGE Enterobacter hormaechei

Organism

Organism UniProt Comment Textmining
Enterobacter hormaechei B6E9W1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTAcolumn chromatography Enterobacter hormaechei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.5
-
purified enzyme, at 37°C Enterobacter hormaechei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose
-
Enterobacter hormaechei alpha,alpha-trehalose
-
?

Synonyms

Synonyms Comment Organism
Trehalose synthase
-
Enterobacter hormaechei
TreS
-
Enterobacter hormaechei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Enterobacter hormaechei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Enterobacter hormaechei