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Literature summary for 5.4.99.16 extracted from
- Role of the C-terminal domain of Thermus thermophilus trehalose synthase in the thermophilicity, thermostability, and efficient production of trehalose (2007), J. Agric. Food Chem., 55, 3435-3443.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Deinococcus radiodurans |
| expression in Escherichia coli | Thermus thermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | deletion mutant lacking 415 amino acids from C-terminus has a lower thermostability and produces more byproducts than wild-type. Fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus has a higher thermostability than Deinococcus radiodurans wild-type and less byproducts | Thermus thermophilus |
| additional information | fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus has a higher thermostability than Deinococcus radiodurans wild-type and less byproducts | Deinococcus radiodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Deinococcus radiodurans |  |
| Hg2+ | - |
Deinococcus radiodurans | |
| Pb2+ | - |
Deinococcus radiodurans | |
| Zn2+ | - |
Deinococcus radiodurans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60700 | - |
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Deinococcus radiodurans |
| 61000 | - |
x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Thermus thermophilus |
| 61000 | - |
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Deinococcus radiodurans |
| 106000 | - |
x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Thermus thermophilus |
| 106000 | - |
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Deinococcus radiodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | - |
- |
- |
| Thermus thermophilus | Q7WUI5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
Thermus thermophilus | alpha,alpha-trehalose | wild-type, 80% yield | ? | |
| maltose | - |
Deinococcus radiodurans | alpha,alpha-trehalose | wild-type, 92% yield | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Thermus thermophilus |
| ? | x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Deinococcus radiodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
wild-type | Deinococcus radiodurans |
| 40 | - |
both deletion mutant lacking 415 amino acids from C-terminus, and fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus | Thermus thermophilus |
| 40 | - |
fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus | Deinococcus radiodurans |
| 65 | - |
wild-type | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, wild-type, almost complete loss of activity, fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, 83% residual activity | Deinococcus radiodurans |
| 80 | - |
30 min, wild-type, 88% residual activity, fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, 83% residual activity | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Deinococcus radiodurans |
| 6.5 | - |
- |
Thermus thermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | wild-type | Deinococcus radiodurans |
| 6 | 9 | - |
Thermus thermophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Deinococcus radiodurans | calculated | - |
4.9 |
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