Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.16 extracted from

  • Ohguchi, M.; Kubota, N.; Wada, T.; Yoshinaga, K.; Uritani, M.; Yagisawa, M.; Ohishi, K.; Yamagishi, M.; Ohta, T.; Ishikawa, K.
    Purification and properties of trehalose-synthesizing enzyme from Pseudomonas sp. F1 (1997), J. Ferment. Bioeng., 84, 358-360.
No PubMed abstract available
Search for more literature for 5.4.99.16

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 maltose
-
 Pseudomonas sp.
26
-
 trehalose
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 4 * 67000, SDS-PAGE Pseudomonas sp.
250000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. F1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.2
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose
-
 Pseudomonas sp. alpha,alpha-trehalose
-
 ?
maltose
-
 Pseudomonas sp. F1 alpha,alpha-trehalose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 67000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 pH 7.0, 1 h, stable below Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 37°C, 1 h, stable Pseudomonas sp.