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Literature summary for 5.4.99.15 extracted from
- Cloning, expression and characterization of the maltooligosyl trehalose synthase from the archaeon Sulfolobus tokodaii (2018), Pak. J. Pharm. Sci., 31, 599-601 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene treY, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 CodonPlus(DE3)-RIL | Sulfurisphaera tokodaii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Lineweaver-Burk kinetics | Sulfurisphaera tokodaii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-[(1->4)-alpha-D-glucosyl]n-1-D-glucose | Sulfurisphaera tokodaii | - |
1-alpha-D-[(1->4)-alpha-D-glucosyl]n-1-alpha-D-glucopyranoside | - |
? |  |
| 4-[(1->4)-alpha-D-glucosyl]n-1-D-glucose | Sulfurisphaera tokodaii DSM 16993 / JCM 10545 / NBRC 100140 / 7 | - |
1-alpha-D-[(1->4)-alpha-D-glucosyl]n-1-alpha-D-glucopyranoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | Q973H2 | i.e. Sulfurisphaera tokodaii | - |
| Sulfurisphaera tokodaii DSM 16993 / JCM 10545 / NBRC 100140 / 7 | Q973H2 | i.e. Sulfurisphaera tokodaii | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 CodonPlus(DE3)-RIL by nickel affinity chromatography to homogeneity | Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.51 U/ml and 0.56 U/ml with maltodextrin and maltopentaose as substrates | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-[(1->4)-alpha-D-glucosyl]n-1-D-glucose | - |
Sulfurisphaera tokodaii | 1-alpha-D-[(1->4)-alpha-D-glucosyl]n-1-alpha-D-glucopyranoside | - |
? | |
| 4-[(1->4)-alpha-D-glucosyl]n-1-D-glucose | - |
Sulfurisphaera tokodaii DSM 16993 / JCM 10545 / NBRC 100140 / 7 | 1-alpha-D-[(1->4)-alpha-D-glucosyl]n-1-alpha-D-glucopyranoside | - |
? | |
| additional information | the archaeal enzyme catalyzes an intramolecular transglycosylation reaction and converts the glycosidic bond at the reducing end of dextrins from alpha-1,4 (reducing end) into alpha-1,1 (non-reducing end). Maltodextrin and maltooligosaccharide are used as substrates by the enzyme but maltose, chitooligosaccharide, sucrose and beta-cyclodextrin are no substrates | Sulfurisphaera tokodaii | ? | - |
? | |
| additional information | the archaeal enzyme catalyzes an intramolecular transglycosylation reaction and converts the glycosidic bond at the reducing end of dextrins from alpha-1,4 (reducing end) into alpha-1,1 (non-reducing end). Maltodextrin and maltooligosaccharide are used as substrates by the enzyme but maltose, chitooligosaccharide, sucrose and beta-cyclodextrin are no substrates | Sulfurisphaera tokodaii DSM 16993 / JCM 10545 / NBRC 100140 / 7 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 83600, recombinant His-tagged enzyme, SDS-PAGE | Sulfurisphaera tokodaii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| maltooligosyl trehalose synthase | - |
Sulfurisphaera tokodaii |
| MTSase | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
recombinant enzyme | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 85 | purified recombinant enzyme, pH 5.0, 10 h, highly stable | Sulfurisphaera tokodaii |
| 95 | - |
purified recombinant enzyme, pH 5.0, 8 h, 60% activity remaining | Sulfurisphaera tokodaii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant enzyme | Sulfurisphaera tokodaii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
purified recombinant enzyme, 75°C, 10 h, 65% activity remaining | Sulfurisphaera tokodaii |
| 4 | - |
purified recombinant enzyme, 75°C, 10 h, 90% activity remaining | Sulfurisphaera tokodaii |
| 10 | 11 | purified recombinant enzyme, 75°C, 10 h, highly stable | Sulfurisphaera tokodaii |
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