Literature summary for 5.4.99.15 extracted from

Tseng, W.C.; Lin, C.R.; Hung, X.G.;, Wei, T.Y.; Chen, Y.C.; Fang, T.Y.
Identification of substrate-binding and selectivity-related residues of maltooligosyltrehalose synthase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092 (2014), Enzyme Microb. Technol., 56, 53-59.

Search for more literature for 5.4.99.15

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant enzymes are expressed in Escherichia coli Rosetta (DE3)	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
A406	compared with wild-type enzyme, the hydrolysis:transglycosylation selectivity ratio is significantly increased	Saccharolobus solfataricus
A406S	the hydrolysis/transglycosylation selectivity ratio shows little change	Saccharolobus solfataricus
D411A	significant reductions in catalytic efficiency, increase in the transition-state energy	Saccharolobus solfataricus
D411A	significant reductions in catalytic efficiency and increase in the transition-state energy	Saccharolobus solfataricus
D610A	significant reductions in catalytic efficiency, increase in the transition-state energy	Saccharolobus solfataricus
D610A	significant reductions in catalytic efficiency and increase in the transition-state energy	Saccharolobus solfataricus
P402	compared with wild-type enzyme, the hydrolysis:transglycosylation selectivity ratio is significantly decreased	Saccharolobus solfataricus
P402Q	the hydrolysis/transglycosylation selectivity ratio is significantly decreased	Saccharolobus solfataricus
R614A	significant reductions in catalytic efficiency, increase in the transition-state energy	Saccharolobus solfataricus
R614A	significant reductions in catalytic efficiency and increase in the transition-state energy	Saccharolobus solfataricus
V426	little change in hydrolysis:transglycosylation selectivity ratio	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.6	-	maltopentaose	pH 5, 60°C, wild-type enzyme	Saccharolobus solfataricus
6.6	-	maltopentaose	pH 5.0, 60°C, wild-type enzyme	Saccharolobus solfataricus
9.74	-	maltopentaose	pH 5.0, 60°C, mutant enzyme P402Q	Saccharolobus solfataricus
10.1	-	maltopentaose	pH 5.0, 60°C, mutant enzyme A406S	Saccharolobus solfataricus
12.6	-	maltopentaose	pH 5.0, 60°C, mutant enzyme V426T	Saccharolobus solfataricus
43.2	-	maltopentaose	pH 5, 60°C, mutant enzyme D411A	Saccharolobus solfataricus
43.2	-	maltopentaose	pH 5.0, 60°C, mutant enzyme D411A	Saccharolobus solfataricus
43.5	-	maltopentaose	pH 5, 60°C, mutant enzyme R614A	Saccharolobus solfataricus
43.5	-	maltopentaose	pH 5.0, 60°C, mutant enzyme R614A	Saccharolobus solfataricus
122	-	maltopentaose	pH 5, 60°C, mutant enzyme D610A	Saccharolobus solfataricus
122	-	maltopentaose	pH 5.0, 60°C, mutant enzyme D610A	Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Saccharolobus solfataricus	key enzyme in the synthesis of trehalose	?	-	?
additional information	Saccharolobus solfataricus P2	key enzyme in the synthesis of trehalose	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-
Saccharolobus solfataricus	Q7LX98	-	-
Saccharolobus solfataricus ATCC 35092	-	-	-
Saccharolobus solfataricus P2	Q7LX98	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Maltopentaose	the enzyme also cytalyzes the hydrolysis of maltopentaose	Saccharolobus solfataricus	alpha-Maltotriosyl trehalose	i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp	?
Maltopentaose	the enzyme also cytalyzes the hydrolysis of maltopentaose	Saccharolobus solfataricus P2	alpha-Maltotriosyl trehalose	i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp	?
maltopentaose	-	Saccharolobus solfataricus	?	-	?
maltopentaose	-	Saccharolobus solfataricus ATCC 35092	?	-	?
additional information	key enzyme in the synthesis of trehalose	Saccharolobus solfataricus	?	-	?
additional information	key enzyme in the synthesis of trehalose	Saccharolobus solfataricus P2	?	-	?

Synonyms

Synonyms	Comment	Organism
maltooligosyltrehalose synthase	-	Saccharolobus solfataricus
MTSase	-	Saccharolobus solfataricus
SSO2095	locus name	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
13.4	-	maltopentaose	pH 5, 60°C, mutant enzyme R614A	Saccharolobus solfataricus
13.4	-	maltopentaose	pH 5.0, 60°C, mutant enzyme R614A	Saccharolobus solfataricus
30.2	-	maltopentaose	pH 5, 60°C, mutant enzyme D411A	Saccharolobus solfataricus
30.2	-	maltopentaose	pH 5.0, 60°C, mutant enzyme D411A	Saccharolobus solfataricus
87.2	-	maltopentaose	pH 5, 60°C, mutant enzyme D610A	Saccharolobus solfataricus
87.2	-	maltopentaose	pH 5.0, 60°C, mutant enzyme D610A	Saccharolobus solfataricus
280	-	maltopentaose	pH 5.0, 60°C, mutant enzyme P402Q	Saccharolobus solfataricus
357	-	maltopentaose	pH 5.0, 60°C, mutant enzyme V426T	Saccharolobus solfataricus
411	-	maltopentaose	pH 5, 60°C, wild-type enzyme	Saccharolobus solfataricus
411	-	maltopentaose	pH 5.0, 60°C, wild-type enzyme	Saccharolobus solfataricus
530	-	maltopentaose	pH 5.0, 60°C, mutant enzyme A406S	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	assay at	Saccharolobus solfataricus

General Information

General Information	Comment	Organism
physiological function	key enzyme in the synthesis of trehalose	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.31	-	maltopentaose	pH 5, 60°C, mutant enzyme R614A	Saccharolobus solfataricus
0.31	-	maltopentaose	pH 5.0, 60°C, mutant enzyme R614A	Saccharolobus solfataricus
0.7	-	maltopentaose	pH 5, 60°C, mutant enzyme D411A	Saccharolobus solfataricus
0.7	-	maltopentaose	pH 5.0, 60°C, mutant enzyme D411A	Saccharolobus solfataricus
0.71	-	maltopentaose	pH 5, 60°C, mutant enzyme D610A	Saccharolobus solfataricus
0.71	-	maltopentaose	pH 5.0, 60°C, mutant enzyme D610A	Saccharolobus solfataricus
28.3	-	maltopentaose	pH 5.0, 60°C, mutant enzyme V426T	Saccharolobus solfataricus
28.8	-	maltopentaose	pH 5.0, 60°C, mutant enzyme P402Q	Saccharolobus solfataricus
52.5	-	maltopentaose	pH 5.0, 60°C, mutant enzyme A406S	Saccharolobus solfataricus
62.3	-	maltopentaose	pH 5, 60°C, wild-type enzyme	Saccharolobus solfataricus
62.3	-	maltopentaose	pH 5.0, 60°C, wild-type enzyme	Saccharolobus solfataricus

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Release 2023.1 (January 2023)