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Literature summary for 5.4.99.15 extracted from
- Trehalose production with a new enzymatic system from Sulfolobus solfataricus KM1 (1999), J. Mol. Catal. B, 6, 223-233.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | trehalose production from starch | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
maltoheptaose | pH 5.5, 60°C | Saccharolobus solfataricus |  |
| 2.7 | - |
maltohexaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 5 | - |
maltopentaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 21.8 | - |
maltotetraose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 228 | - |
maltotriose | pH 5.5, 60°C | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
x * 76000, SDS-PAGE | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus KM1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltoheptaose | - |
Saccharolobus solfataricus | alpha-maltopentaosyl trehalose | - |
? | |
| maltoheptaose | - |
Saccharolobus solfataricus KM1 | alpha-maltopentaosyl trehalose | - |
? | |
| maltohexaose | - |
Saccharolobus solfataricus | alpha-maltotetraosyltrehalose | - |
? | |
| maltohexaose | - |
Saccharolobus solfataricus KM1 | alpha-maltotetraosyltrehalose | - |
? | |
| maltopentaose | - |
Saccharolobus solfataricus | alpha-maltotriosyltrehalose | - |
? | |
| maltopentaose | - |
Saccharolobus solfataricus KM1 | alpha-maltotriosyltrehalose | - |
? | |
| maltotetraose | - |
Saccharolobus solfataricus | alpha-maltosyltrehalose | - |
? | |
| maltotetraose | - |
Saccharolobus solfataricus KM1 | alpha-maltosyltrehalose | - |
? | |
| maltotriose | - |
Saccharolobus solfataricus | alpha-glucosyltrehalose | - |
? | |
| additional information | no activity with maltose. The enzyme never produces a glycosyltrehalose of longer chain length than the substrate used. Glucose and maltooligosaccharide shortened by one glucose residue are produced as by-products | Saccharolobus solfataricus | ? | - |
? | |
| additional information | no activity with maltose. The enzyme never produces a glycosyltrehalose of longer chain length than the substrate used. Glucose and maltooligosaccharide shortened by one glucose residue are produced as by-products | Saccharolobus solfataricus KM1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
6 h, 9% loss of activity | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Saccharolobus solfataricus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | stable | Saccharolobus solfataricus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | isoelectric focusing | - |
6.1 |
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Release 2023.1 (January 2023)
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