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Literature summary for 5.4.99.15 extracted from

  • Kato, M.; Miura, Y.; Kettoku, M.; Shindo, K.; Iwamatsu, A.; Kobayashi, K.
    Reaction mechanism of a new glycosyltrehalose-producing enzyme isolated from the hyperthermophilic archaeum, Sulfolobus solfataricus KM1 (1996), Biosci. Biotechnol. Biochem., 60, 921-924.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 5 mM Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
maltoheptaose pH 5.5, 60°C Saccharolobus solfataricus
2.7
-
maltohexaose pH 5.5, 60°C Saccharolobus solfataricus
5
-
maltopentaose pH 5.5, 60°C Saccharolobus solfataricus
21.8
-
maltotetraose pH 5.5, 60°C Saccharolobus solfataricus
228
-
maltotriose pH 5.5, 60°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus KM1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose 88.6% of the activity compared to maltopentaose Saccharolobus solfataricus alpha-maltopentaosyl trehalose
-
?
maltoheptaose 88.6% of the activity compared to maltopentaose Saccharolobus solfataricus KM1 alpha-maltopentaosyl trehalose
-
?
maltohexaose 80% of the activity compared to maltopentaose Saccharolobus solfataricus alpha-maltotetraosyltrehalose
-
?
maltohexaose 80% of the activity compared to maltopentaose Saccharolobus solfataricus KM1 alpha-maltotetraosyltrehalose
-
?
maltopentaose
-
Saccharolobus solfataricus alpha-maltotriosyltrehalose
-
?
maltopentaose
-
Saccharolobus solfataricus KM1 alpha-maltotriosyltrehalose
-
?
maltotetraose 46.3% of the activity compared to maltopentaose Saccharolobus solfataricus alpha-maltosyltrehalose
-
?
maltotetraose 46.3% of the activity compared to maltopentaose Saccharolobus solfataricus KM1 alpha-maltosyltrehalose
-
?
maltotriose 5.0% of the activity compared to maltopentaose Saccharolobus solfataricus alpha-glucosyltrehalose
-
?
maltotriose 5.0% of the activity compared to maltopentaose Saccharolobus solfataricus KM1 alpha-glucosyltrehalose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Saccharolobus solfataricus
70 80
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
6 h, 9% loss of activity Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
Saccharolobus solfataricus
5.5
-
assay at Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 stable Saccharolobus solfataricus