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Literature summary for 5.4.99.15 extracted from

  • Lee, J.S.; Hai, T.; Pape, H.; Kim, T.J.; Suh, J.W.
    Three trehalose synthetic pathways in the acarbose-producing Actinoplanes sp. SN223/29 and evidence for the TreY role in biosynthesis of component C (2008), Appl. Microbiol. Biotechnol., 80, 767-778.
    View publication on PubMed
Search for more literature for 5.4.99.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Actinoplanes sp.

Organism

Organism UniProt Comment Textmining
Actinoplanes sp.
-
-
-
Actinoplanes sp. SN223/29
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Actinoplanes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acarbose
-
 Actinoplanes sp. ?
-
 ?
acarbose
-
 Actinoplanes sp. SN223/29 ?
-
 ?
maltohexaose
-
 Actinoplanes sp. alpha-maltotetraosyl trehalose
-
 ?
maltohexaose
-
 Actinoplanes sp. SN223/29 alpha-maltotetraosyl trehalose
-
 ?

Synonyms

Synonyms Comment Organism
maltooligosyltrehalose synthase
-
 Actinoplanes sp.
TreY
-
 Actinoplanes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 in 100 mM phosphate buffer Actinoplanes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in 100 mM phosphate buffer Actinoplanes sp.