Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.15 extracted from

  • Seo, J.S.; An, J.H.; Baik, M.Y.; Park, C.S.; Cheong, J.J.; Moon, T.W.; Park, K.H.; Choi, Y.D.; Kim, C.H.
    Molecular cloning and characterization of trehalose biosynthesis genes from hyperthermophilic archaebacterium Metallosphaera hakonesis (2007), J. Microbiol. Biotechnol., 17, 123-129.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of trehalose by enzyme plus maltooligosyltrehalose trehalohydrolase is increased in presence of 4-alpha-glucanotransferase, converting 70% of maltopentaose into trehalose after 6 h, while 60% is converted without 4-alpha-glucanotransferase Metallosphaera hakonensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Metallosphaera hakonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83800
-
x * 83800, calculated, x * 90000, SDS-PAGE Metallosphaera hakonensis
90000
-
x * 83800, calculated, x * 90000, SDS-PAGE Metallosphaera hakonensis

Organism

Organism UniProt Comment Textmining
Metallosphaera hakonensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
liquified corn starch
-
Metallosphaera hakonensis ? in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose ?
liquified corn starch
-
Metallosphaera hakonensis JCM 8857 ? in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose ?
Maltopentaose
-
Metallosphaera hakonensis alpha-Maltotriosyl trehalose
-
?
Maltopentaose
-
Metallosphaera hakonensis JCM 8857 alpha-Maltotriosyl trehalose
-
?
soluble starch
-
Metallosphaera hakonensis ? in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose ?
soluble starch
-
Metallosphaera hakonensis JCM 8857 ? in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose ?

Subunits

Subunits Comment Organism
? x * 83800, calculated, x * 90000, SDS-PAGE Metallosphaera hakonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Metallosphaera hakonensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
48 h, 80% residual activity Metallosphaera hakonensis
85
-
activity is gradually abolished Metallosphaera hakonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Metallosphaera hakonensis