Application | Comment | Organism |
---|---|---|
synthesis | production of trehalose by enzyme plus maltooligosyltrehalose trehalohydrolase is increased in presence of 4-alpha-glucanotransferase, converting 70% of maltopentaose into trehalose after 6 h, while 60% is converted without 4-alpha-glucanotransferase | Metallosphaera hakonensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Metallosphaera hakonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83800 | - |
x * 83800, calculated, x * 90000, SDS-PAGE | Metallosphaera hakonensis |
90000 | - |
x * 83800, calculated, x * 90000, SDS-PAGE | Metallosphaera hakonensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera hakonensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
liquified corn starch | - |
Metallosphaera hakonensis | ? | in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose | ? | |
liquified corn starch | - |
Metallosphaera hakonensis JCM 8857 | ? | in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose | ? | |
Maltopentaose | - |
Metallosphaera hakonensis | alpha-Maltotriosyl trehalose | - |
? | |
Maltopentaose | - |
Metallosphaera hakonensis JCM 8857 | alpha-Maltotriosyl trehalose | - |
? | |
soluble starch | - |
Metallosphaera hakonensis | ? | in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose | ? | |
soluble starch | - |
Metallosphaera hakonensis JCM 8857 | ? | in presence of maltooligosyltrehalose trehalohydrolase, conversion of soluble starch into trehalose | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83800, calculated, x * 90000, SDS-PAGE | Metallosphaera hakonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Metallosphaera hakonensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
48 h, 80% residual activity | Metallosphaera hakonensis |
85 | - |
activity is gradually abolished | Metallosphaera hakonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Metallosphaera hakonensis |