Literature summary for 5.4.99.15 extracted from

Fang, T.; Tseng, W.; Chung, Y.; Pan, C.
Mutations on aromatic residues of the active site to alter selectivity of the Sulfolobus solfataricus maltooligosyltrehalose synthase (2006), J. Agric. Food Chem., 54, 3585-3590.

Search for more literature for 5.4.99.15

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
F405Y	mutation located near subsite +1 constructed to alter enzyme selectivity. Catalytic efficiency for transglycosylation reaction is similar to wild-type, for hydrolysis somewhat lower than wild-type	Saccharolobus solfataricus
Y290F	mutation located near subsite +1 constructed to alter enzyme selectivity. Catalytic efficiency for hydrolysis and transglycosylation reaction are 6.6 and 5.6% resp., of wild-type	Saccharolobus solfataricus
Y367F	mutation located near subsite +1 constructed to alter enzyme selectivity. Catalytic efficiency for hydrolysis and transglycosylation reaction are about half of wild-type	Saccharolobus solfataricus
Y409F	mutation located near subsite +1 constructed to alter enzyme selectivity. Catalytic efficiency for hydrolysis is similar to wild-type, for transglycosylation reaction somewhat lower than wild-type	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.78	-	maltohexaose	mutant Y367F, pH 5.0, 60°C	Saccharolobus solfataricus
3.8	-	maltoheptaose	mutant F405Y, pH 5.0, 60°C	Saccharolobus solfataricus
3.8	-	maltoheptaose	mutant Y409F, pH 5.0, 60°C	Saccharolobus solfataricus
3.88	-	maltoheptaose	wild-type, pH 5.0, 60°C	Saccharolobus solfataricus
4.53	-	maltohexaose	wild-type, pH 5.0, 60°C	Saccharolobus solfataricus
4.9	-	maltohexaose	mutant F405Y, pH 5.0, 60°C	Saccharolobus solfataricus
5.85	-	maltopentaose	mutant Y409F, pH 5.0, 60°C	Saccharolobus solfataricus
5.94	-	maltopentaose	wild-type, pH 5.0, 60°C	Saccharolobus solfataricus
6.26	-	maltohexaose	mutant Y409F, pH 5.0, 60°C	Saccharolobus solfataricus
6.34	-	maltopentaose	mutant F405Y, pH 5.0, 60°C	Saccharolobus solfataricus
11.6	-	maltotetraose	mutant F405Y, pH 5.0, 60°C	Saccharolobus solfataricus
13.2	-	maltotetraose	wild-type, pH 5.0, 60°C	Saccharolobus solfataricus
16.1	-	maltotetraose	mutant Y409F, pH 5.0, 60°C	Saccharolobus solfataricus
26.5	-	maltohexaose	mutant Y290F, pH 5.0, 60°C	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutants	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltoheptaose	-	Saccharolobus solfataricus	alpha-maltopentaosyl trehalose	-	?
maltohexaose	-	Saccharolobus solfataricus	alpha-maltotetraosyl trehalose	-	?
Maltopentaose	-	Saccharolobus solfataricus	alpha-Maltotriosyl trehalose	-	?
Maltotetraose	-	Saccharolobus solfataricus	?	-	?

Synonyms

Synonyms	Comment	Organism
maltooligosyltrehalose synthase	-	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
26.5	-	maltohexaose	mutant Y290F, pH 5.0, 60°C	Saccharolobus solfataricus
114	-	maltotetraose	mutant Y409F, pH 5.0, 60°C	Saccharolobus solfataricus
138	-	maltotetraose	wild-type, pH 5.0, 60°C	Saccharolobus solfataricus
139	-	maltotetraose	mutant F405Y, pH 5.0, 60°C	Saccharolobus solfataricus
139	-	maltohexaose	mutant Y367F, pH 5.0, 60°C	Saccharolobus solfataricus
267	-	maltopentaose	mutant Y409F, pH 5.0, 60°C	Saccharolobus solfataricus
282	-	maltoheptaose	mutant Y409F, pH 5.0, 60°C	Saccharolobus solfataricus
330	-	maltohexaose	mutant Y409F, pH 5.0, 60°C	Saccharolobus solfataricus
354	-	maltopentaose	mutant F405Y, pH 5.0, 60°C	Saccharolobus solfataricus
359	-	maltohexaose	wild-type, pH 5.0, 60°C	Saccharolobus solfataricus
362	-	maltohexaose	mutant F405Y, pH 5.0, 60°C	Saccharolobus solfataricus
363	-	maltoheptaose	mutant F405Y, pH 5.0, 60°C	Saccharolobus solfataricus
364	-	maltopentaose	wild-type, pH 5.0, 60°C	Saccharolobus solfataricus
383	-	maltoheptaose	wild-type, pH 5.0, 60°C	Saccharolobus solfataricus

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Release 2023.1 (January 2023)