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Literature summary for 5.4.99.15 extracted from

  • Gueguen, Y.; Rolland, J.L.; Schroeck, S.; Flament, D.; Defretin, S.; Saniez, M.H.; Dietrich, J.
    Characterization of the maltooligosyl trehalose synthase from the thermophilic archaeon Sulfolobus acidocaldarius (2001), FEMS Microbiol. Lett., 194, 201-206.
    View publication on PubMed
Search for more literature for 5.4.99.15

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli using the T7-expression system Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Maltoheptaose
-
 Sulfolobus acidocaldarius ?
-
 ?
maltopentaose
-
 Sulfolobus acidocaldarius ?
-
 ?
Maltotetraose
-
 Sulfolobus acidocaldarius ?
-
 ?
maltotriose
-
 Sulfolobus acidocaldarius ?
-
 ?
additional information possible mechanism of action: after converting the alpha-1,4-glucosidic linkage to an alpha-1,1-glucosidic linkage at the reducing end of maltooligosaccharide Glc(n) is able to release glucose and maltooligosaccharide Glc(n-1) residues. Then the intramolecular transglycosylation and the hydrolytic reaction continues with maltooligosaccharide Glc(n-1) until the initial maltooligosaccharide is reduced to maltose Sulfolobus acidocaldarius ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 72 h, citrate-phosphate buffer, 38% loss of activity Sulfolobus acidocaldarius
90
-
 1 h, 30% loss of activity Sulfolobus acidocaldarius
100
-
 1 h, 65% loss of activity Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Sulfolobus acidocaldarius