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Literature summary for 5.4.99.14 extracted from
- Characterization of a gene cluster from Ralstonia eutropha JMP134 encoding metabolism of 4-methylmuconolactone (1998), Gene, 206, 53-62.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mmlI, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Cupriavidus necator |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | inhibition is reversible by DTT | Cupriavidus necator |  |
| Cu2+ | - |
Cupriavidus necator | |
| additional information | no inhibition by EDTA | Cupriavidus necator |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12900 | - |
2 * 12900, about, sequence calculation | Cupriavidus necator |
| 25000 | - |
gel filtration | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methyl-muconolactone | Cupriavidus necator | - |
3-methyl-muconolactone | - |
r | |
| 4-methyl-muconolactone | Cupriavidus necator JMP 134-1 | - |
3-methyl-muconolactone | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | O51799 | gene mmlI | - |
| Cupriavidus necator JMP 134-1 | O51799 | gene mmlI | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (4R,5R)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | trans-3-methyldienelactone | - |
r | |
| (4R,5R)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator JMP 134-1 | trans-3-methyldienelactone | - |
r | |
| (4S,5S)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator | trans-3-methyldienelactone | - |
r | |
| (4S,5S)-5-chloro-3-methylmuconolactone | - |
Cupriavidus necator JMP 134-1 | trans-3-methyldienelactone | - |
r | |
| 4-methyl-muconolactone | - |
Cupriavidus necator | 3-methyl-muconolactone | - |
r | |
| 4-methyl-muconolactone | - |
Cupriavidus necator JMP 134-1 | 3-methyl-muconolactone | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 12900, about, sequence calculation | Cupriavidus necator |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-Methylmuconolactone methylisomerase | - |
Cupriavidus necator |
| MLMI | - |
Cupriavidus necator |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Cupriavidus necator |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the modified ortho-cleavage pathway and methylmuconolactone degradation of Ralstonia eutropha strain JMP134 | Cupriavidus necator |
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