Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paraburkholderia xenovorans |
expressed in Escherichia coli BL21(DE3) cells | Geobacillus kaustophilus |
expressed in Escherichia coli BL21(DE3) cells | Nocardia farcinica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | the presence of GDP decreases kcat about 1.6-1.7fold while increasing the Km about 2fold. Consequently, the catalytic efficiency kcat/Km value of IcmF decreases 3fold in the presence of GDP | Geobacillus kaustophilus | |
GTP | the presence of GTP decreases kcat about 1.6-1.7fold while increasing the Km about 2fold. Consequently, the catalytic efficiency kcat/Km value of IcmF decreases 4.5fold in the presence of GTP | Geobacillus kaustophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0201 | - |
isobutyryl-CoA | recombinant enzyme, at 37°C | Geobacillus kaustophilus | |
0.0453 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
0.0508 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | - |
- |
- |
Nocardia farcinica | - |
- |
- |
Paraburkholderia xenovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration | Paraburkholderia xenovorans |
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration | Geobacillus kaustophilus |
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration | Nocardia farcinica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
recombinant enzyme, at 37°C | Paraburkholderia xenovorans |
0.75 | - |
recombinant enzyme, at 37°C | Geobacillus kaustophilus |
1.1 | - |
recombinant enzyme, at 37°C | Nocardia farcinica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isobutyryl-CoA | reversible interconversion | Paraburkholderia xenovorans | n-butyryl-CoA | - |
r | |
isobutyryl-CoA | reversible interconversion | Geobacillus kaustophilus | n-butyryl-CoA | - |
r | |
isobutyryl-CoA | reversible interconversion | Nocardia farcinica | n-butyryl-CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ICM | - |
Paraburkholderia xenovorans |
ICM | - |
Geobacillus kaustophilus |
ICM | - |
Nocardia farcinica |
IcmF | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Paraburkholderia xenovorans |
IcmF | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Geobacillus kaustophilus |
IcmF | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Nocardia farcinica |
isobutyryl-CoA mutase fused | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Paraburkholderia xenovorans |
isobutyryl-CoA mutase fused | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Geobacillus kaustophilus |
isobutyryl-CoA mutase fused | a fusion between isobutyryl-CoA mutase and its G-protein chaperone | Nocardia farcinica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme is unstable at 60°C | Geobacillus kaustophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.88 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
1.96 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
3.1 | - |
isobutyryl-CoA | recombinant enzyme, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
43 | - |
isobutyryl-CoA | recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C | Geobacillus kaustophilus | |
150 | - |
isobutyryl-CoA | recombinant enzyme, at 37°C | Geobacillus kaustophilus |