Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D151A | inactive | Saccharomyces cerevisiae |
D151A | inactive mutant | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine38 | Saccharomyces cerevisiae | - |
tRNA pseudouridine38 | - |
? | |
tRNA uridine39 | Saccharomyces cerevisiae | - |
tRNA pseudouridine39 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P31115 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine38 | - |
Saccharomyces cerevisiae | tRNA pseudouridine38 | - |
? | |
tRNA uridine39 | - |
Saccharomyces cerevisiae | tRNA pseudouridine39 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PUS3 | - |
Saccharomyces cerevisiae |
tRNA pseudouridine synthase 3 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the PUS3 gene has an effect on the efficiency of the translation process. Reduced readthrough efficiency of each stop codon by natural nonsense suppressor tRNAs | Saccharomyces cerevisiae |
physiological function | deletion of the PUS3 gene, encoding the enzyme that introduces pseudouridines at position 38 or 39 in tRNA, has an effect on the efficiency of the translation process. In the mutant, there is a reduced readthrough efficiency of each stop codon by natural nonsense suppressor tRNAs. This effect is solely due to the absence of pseudouridine 38 or 39 in tRNA. The presence of pseudouridine 38 or 39 in the tRNA anticodon arm enhances misreading of certain codons by natural nonsense tRNAs as well as promotes frameshifting on slippery sequences in yeast | Saccharomyces cerevisiae |