Literature summary for 5.4.99.11 extracted from

Lipski, A.; Watzlawick, H.; Ravaud, S.; Robert, X.; Rhimi, M.; Haser, R.; Mattes, R.; Aghajari, N.
Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity (2013), Acta Crystallogr. Sect. D, 69, 298-307.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene mutB, recombinant expression of wild-type and mutant enzymes	Rhizobium sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme mutants R284C in complex with transition-state mimic deoxynojirimycin, and F164L in complex with glucose, sucrose, trehalulose, or isomaltulose, X-ray diffraction structure determination and analysis at 1.7-2.15 A resolution	Rhizobium sp.

Protein Variants

Protein Variants	Comment	Organism
F164L	site-directed mutagenesis, three-dimensional structure from crystal structure analysis, comparison to the wild-type. The mutant shows hydrolytic activity converting sucrose to glucose and fructose, kinetics	Rhizobium sp.
R284C	site-directed mutagenesis, three-dimensional structure from crystal structure analysis, comparison to the wild-type. The mutant shows hydrolytic activity converting sucrose to glucose and fructose, kinetics. Presence of Mg2+, Ca2+ and Zn2+ ions and glucose have no effect on the activity of the R284C mutant	Rhizobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rhizobium sp.	the enzyme catalyzes the isomerization of sucrose (alpha-D-glucosylpyranosyl-1,2-beta-D-fructofuranose) to trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) and isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) as the main products, and produces glucose and fructose in residual amounts because of sucrose hydrolysis	?	-	?
sucrose	Rhizobium sp.	-	1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose	main products	?

Organism

Organism	UniProt	Comment	Textmining
Rhizobium sp.	M1E1F6	gene mutB	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes	Rhizobium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme catalyzes the isomerization of sucrose (alpha-D-glucosylpyranosyl-1,2-beta-D-fructofuranose) to trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) and isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) as the main products, and produces glucose and fructose in residual amounts because of sucrose hydrolysis	Rhizobium sp.	?	-	?
sucrose	-	Rhizobium sp.	1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose	main products	?

Synonyms

Synonyms	Comment	Organism
MutB	-	Rhizobium sp.
sucrose isomerase	-	Rhizobium sp.

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycoside hydrolase family 13 (GH13) in the CAZy classification	Rhizobium sp.
additional information	active site structure and substrate and ligand binding structures, overview	Rhizobium sp.

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Release 2023.1 (January 2023)