Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Erwinia rhapontici |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 20-30% inhibition at 1 mM | Erwinia rhapontici | |
Co2+ | 20-30% inhibition at 1 mM | Erwinia rhapontici | |
Cu2+ | 20-30% inhibition at 1 mM | Erwinia rhapontici | |
EDTA | 18% inhibition at 5 mM | Erwinia rhapontici | |
Zn2+ | 20-30% inhibition at 1 mM | Erwinia rhapontici |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
257 | - |
sucrose | in 50 mM PBS (pH 6.0), at 30°C | Erwinia rhapontici |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Erwinia rhapontici | |
additional information | Mg2+ and Zn2+ show no significant influence on enzyme activity | Erwinia rhapontici |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Erwinia rhapontici |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erwinia rhapontici | D9MPF2 | - |
- |
Erwinia rhapontici NX-5 | D9MPF2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Erwinia rhapontici |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | 99% conversion | Erwinia rhapontici | isomaltulose | - |
? | |
sucrose | 99% conversion | Erwinia rhapontici NX-5 | isomaltulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Erwinia rhapontici |
Synonyms | Comment | Organism |
---|---|---|
SIase | - |
Erwinia rhapontici |
sucrose isomerase | - |
Erwinia rhapontici |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Erwinia rhapontici |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme is stable for longer than 48 h at 30°C. The stability of the enzyme decreases drastically at 50°C and 60°C with a half-life duration of 90 min and 5 min, respectively | Erwinia rhapontici |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Erwinia rhapontici |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | 89%, 86%, 80%, 34%, and 30% of maximum activity at pH 4.0, pH 6.0, pH 7.0, pH 8.0, and pH 9.0 | Erwinia rhapontici |