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Literature summary for 5.4.99.11 extracted from

  • Aroonnual, A.; Nihira, T.; Seki, T.; Panbangred, W.
    Role of several key residues in the catalytic activity of sucrose isomerase from Klebsiella pneumoniae NK33-98-8 (2007), Enzyme Microb. Technol., 40, 1221-1227.
No PubMed abstract available
Search for more literature for 5.4.99.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
D140E almost complete loss of catalytic activity Klebsiella pneumoniae
D140G almost complete loss of catalytic activity Klebsiella pneumoniae
D140N almost complete loss of catalytic activity Klebsiella pneumoniae
D329A 42% of wild-type activity, no change in major products Klebsiella pneumoniae
L326Y 20% of wild-type activity, no change in major products Klebsiella pneumoniae
L326Y/D329A 3.4% of wild-type activity, no change in major products Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, complete inhibition Klebsiella pneumoniae
sodium dodecylsulfate 1 mM, complete inhibition Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
42.7
-
 sucrose pH 6.0, 30°C Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 x * 69980, calculated, x * 67000, SDS-PAGE Klebsiella pneumoniae
69980
-
 x * 69980, calculated, x * 67000, SDS-PAGE Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae NK33-98-8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2362
-
 pH 6.0, 30°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme does not catalyze the formation of any disaccharide from glucose and fructose. No trehalulose is detected when palatinose is the substrate and vice versa Klebsiella pneumoniae ?
-
 ?
additional information enzyme does not catalyze the formation of any disaccharide from glucose and fructose. No trehalulose is detected when palatinose is the substrate and vice versa Klebsiella pneumoniae NK33-98-8 ?
-
 ?
sucrose
-
 Klebsiella pneumoniae palatinose + trehalulose + D-glucose + D-fructose at 30°C, 76.8% palatinose, 21.2% trehalulose, 1% each of D-glucose and D-fructose. Percentage of palatinose increases with temperature and reaches a maximum of 79.7% at 40°C ir
sucrose
-
 Klebsiella pneumoniae NK33-98-8 palatinose + trehalulose + D-glucose + D-fructose at 30°C, 76.8% palatinose, 21.2% trehalulose, 1% each of D-glucose and D-fructose. Percentage of palatinose increases with temperature and reaches a maximum of 79.7% at 40°C ir

Subunits

Subunits Comment Organism
? x * 69980, calculated, x * 67000, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 25% residual activity Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable for at least 1 h Klebsiella pneumoniae
50
-
 20 min, loss of 40% of activity Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 80% residual activity Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 1 h, more than 80% residual activity Klebsiella pneumoniae

pI Value

Organism Comment pI Value Maximum pI Value
Klebsiella pneumoniae calculated
-
 5.9