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Literature summary for 5.4.99.11 extracted from
- Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene (2005), Appl. Environ. Microbiol., 71, 1581-1590.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, with His-tag | Pantoea dispersa |
| expression in Escherichia coli, with His-tag | Raoultella planticola |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | ratio of Kcat/KM-value is 0.000062 per M and s | Raoultella planticola | |
| additional information | - |
additional information | ratio of Kcat/KM-value is 0.000179 per M and s | Pantoea dispersa | |
| 39.9 | - |
sucrose | pH 6.0, 30°C | Pantoea dispersa |  |
| 76 | - |
sucrose | pH 6.0, 30°C | Raoultella planticola | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65819 | - |
x * 65819, mature gene product, calculated | Pantoea dispersa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pantoea dispersa | Q6XNK6 | - |
- |
| Pantoea dispersa UQ68J | Q6XNK6 | - |
- |
| Raoultella planticola | Q6XKX6 | - |
- |
| Raoultella planticola UQ14S | Q6XKX6 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | sequence contains a periplasm-targeting leader of 33 amino acids | Pantoea dispersa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 351 | - |
pH 6.0, 30°C | Raoultella planticola |
| 562 | - |
pH 6.0, 30°C | Pantoea dispersa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, stable for at least 6 months | Pantoea dispersa |
| -20°C, 50% glycerol, stable for at least 6 months | Raoultella planticola |
| 22°C, 50% glycerol, 60% residual activity after 15 days | Pantoea dispersa |
| 22°C, 50% glycerol, 60% residual activity after 15 days | Raoultella planticola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no apparent reverse reaction producing glucose, fructose, or trehalose, from isomaltulose | Pantoea dispersa | ? | - |
? | |
| additional information | no apparent reverse reaction producing glucose, fructose, or trehalose, from isomaltulose | Pantoea dispersa UQ68J | ? | - |
? | |
| sucrose | - |
Pantoea dispersa | alpha-D-glucopyranosyl-1,6-D-fructofuranose + alpha-D-glucopyranosyl-1,1-D-fructofuranose | ratio of alpha-D-glucopyranosyl-1,6-D-fructofuranose to alpha-D-glucopyranosyl-1,1-D-fructofuranose produced is about 22:1 across pH- and temperature-range. Reaction is fast, conversion of 97% of sucrose in assay within 45 min | ir | |
| sucrose | - |
Raoultella planticola | alpha-D-glucopyranosyl-1,6-D-fructofuranose + alpha-D-glucopyranosyl-1,1-D-fructofuranose | ratio of alpha-D-glucopyranosyl-1,6-D-fructofuranose to alpha-D-glucopyranosyl-1,1-D-fructofuranose produced is about 8:1 across pH- and temperature-range. Enzyme converts 84% of sucrose present in assay within 2 h | ir | |
| sucrose | - |
Raoultella planticola UQ14S | alpha-D-glucopyranosyl-1,6-D-fructofuranose + alpha-D-glucopyranosyl-1,1-D-fructofuranose | ratio of alpha-D-glucopyranosyl-1,6-D-fructofuranose to alpha-D-glucopyranosyl-1,1-D-fructofuranose produced is about 8:1 across pH- and temperature-range. Enzyme converts 84% of sucrose present in assay within 2 h | ir | |
| sucrose | - |
Pantoea dispersa UQ68J | alpha-D-glucopyranosyl-1,6-D-fructofuranose + alpha-D-glucopyranosyl-1,1-D-fructofuranose | ratio of alpha-D-glucopyranosyl-1,6-D-fructofuranose to alpha-D-glucopyranosyl-1,1-D-fructofuranose produced is about 22:1 across pH- and temperature-range. Reaction is fast, conversion of 97% of sucrose in assay within 45 min | ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65819, mature gene product, calculated | Pantoea dispersa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | - |
Pantoea dispersa |
| 35 | - |
- |
Raoultella planticola |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Pantoea dispersa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
50% glycerol, 60% residual activity after 15 days | Pantoea dispersa |
| 22 | - |
50% glycerol, 60% residual activity after 15 days | Raoultella planticola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Pantoea dispersa |
| 6 | - |
- |
Raoultella planticola |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Pantoea dispersa |
| 4 | 7 | - |
Raoultella planticola |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pantoea dispersa | mature gene product, calculated | - |
7 |
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