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Literature summary for 5.4.4.4 extracted from
- Linalool dehydratase-isomerase, a bifunctional enzyme in the anaerobic degradation of monoterpenes (2010), J. Biol. Chem., 285, 30436-30442.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol | Castellaniella defragrans |  |
| additional information | the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool | Castellaniella defragrans | |
| S-adenosyl-L-methionine | 40 mM, 2fold activation | Castellaniella defragrans | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Castellaniella defragrans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by 10% (v/v) DMSO. EDTA (5 mM) does not affect the enzyme activity | Castellaniella defragrans | |
| O2 | the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool | Castellaniella defragrans | |
| Ti(III)citrate | 1 mM, complete inhibition | Castellaniella defragrans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
geraniol | pH 9.0, 35°C | Castellaniella defragrans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, SDS-PAGE | Castellaniella defragrans |
| 160000 | - |
gel filtration | Castellaniella defragrans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geraniol | Castellaniella defragrans | - |
linalool | - |
r | |
| geraniol | Castellaniella defragrans 65Phen | - |
linalool | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Castellaniella defragrans | E1XUJ2 | - |
- |
| Castellaniella defragrans 65Phen | E1XUJ2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | precursor protein with a signal peptide for a periplasmic location | Castellaniella defragrans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Castellaniella defragrans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geraniol | - |
Castellaniella defragrans | linalool | - |
r | |
| geraniol | the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol. The enzyme catalyses in vitro the reaction in both directions depending on the thermodynamic driving forces | Castellaniella defragrans | linalool | - |
r | |
| geraniol | - |
Castellaniella defragrans 65Phen | linalool | - |
r | |
| geraniol | the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol. The enzyme catalyses in vitro the reaction in both directions depending on the thermodynamic driving forces | Castellaniella defragrans 65Phen | linalool | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Castellaniella defragrans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Castellaniella defragrans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | contains no prosthetic group | Castellaniella defragrans |
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Release 2023.1 (January 2023)
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