Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulates, 2.25fold stimulation at 20-30 mM | Escherichia coli | |
dithiothreitol | stimulates, 2fold stimulation at 1 mM | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
K+ | - |
Escherichia coli | |
Mg2+ | above 1 mM | Escherichia coli | |
NEM | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.195 | - |
chorismate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | sharp increase in activity, optimal concentration: 1 mM, inhibition above 1 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, isochorismate synthase involved in enterobactin biosynthesis, MenF, SDS-PAGE | Escherichia coli |
98000 | - |
isochorismate synthase involved in enterobactin biosynthesis, MenF, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | Escherichia coli | first committed step in the biosynthesis of menaquinone | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isochorismate synthase involved in enterobactin biosynthesis, MenF. The isochorismate synthase involved in menaquinone biosynthesis is different from the isochorismate synthase involved in enterobactin biosynthesis | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.808 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 40% loss of activity after 5 days. 1% bovine serum albumin, 10% glycerol, or 20% dimethyl sulfoxide stabilize for up to 10 days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | not reversible, isochorismate synthase involved in enterobactin biosynthesis, MenF | Escherichia coli | Isochorismate | - |
? | |
Chorismate | first committed step in the biosynthesis of menaquinone | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, isochorismate synthase involved in enterobactin biosynthesis, MenF, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | 15°C: 72% of maximal activity, 20°C: about 74% of maximal activity, 30°C or 40°C: about 80% of maximal activity, 50°C: 42% of maximal activity | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.33 | - |
chorismate | in absence of 2-mercaptoethanol | Escherichia coli | |
2.93 | - |
chorismate | in presence of 2-mercaptoethanol | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | about 20% of maximal activity at pH 6.5 and pH 9.0 | Escherichia coli |
7.5 | 8 | - |
Escherichia coli |