Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.4.2 extracted from

  • Daruwala, R.; Bhattacharyya, D.K.; Kwon, O.; Meganathan, R.
    Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and characterization of a new isochorismate synthase from Escherichia coli (1997), J. Bacteriol., 179, 3133-3138.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.4.2

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates, 2.25fold stimulation at 20-30 mM Escherichia coli
dithiothreitol stimulates, 2fold stimulation at 1 mM Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Escherichia coli
Hg2+
-
 Escherichia coli
K+
-
 Escherichia coli
Mg2+ above 1 mM Escherichia coli
NEM
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.195
-
 chorismate
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ sharp increase in activity, optimal concentration: 1 mM, inhibition above 1 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, isochorismate synthase involved in enterobactin biosynthesis, MenF, SDS-PAGE Escherichia coli
98000
-
 isochorismate synthase involved in enterobactin biosynthesis, MenF, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Chorismate Escherichia coli first committed step in the biosynthesis of menaquinone ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 isochorismate synthase involved in enterobactin biosynthesis, MenF. The isochorismate synthase involved in menaquinone biosynthesis is different from the isochorismate synthase involved in enterobactin biosynthesis
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.808
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 40% loss of activity after 5 days. 1% bovine serum albumin, 10% glycerol, or 20% dimethyl sulfoxide stabilize for up to 10 days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate not reversible, isochorismate synthase involved in enterobactin biosynthesis, MenF Escherichia coli Isochorismate
-
 ?
Chorismate first committed step in the biosynthesis of menaquinone Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, isochorismate synthase involved in enterobactin biosynthesis, MenF, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 15°C: 72% of maximal activity, 20°C: about 74% of maximal activity, 30°C or 40°C: about 80% of maximal activity, 50°C: 42% of maximal activity Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.33
-
 chorismate in absence of 2-mercaptoethanol Escherichia coli
2.93
-
 chorismate in presence of 2-mercaptoethanol Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9 about 20% of maximal activity at pH 6.5 and pH 9.0 Escherichia coli
7.5 8
-
 Escherichia coli