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Literature summary for 5.4.3.6 extracted from

  • Wanninayake, U.; Walker, K.D.
    A bacterial tyrosine aminomutase proceeds through retention or inversion of stereochemistry to catalyze its isomerization reaction (2013), J. Am. Chem. Soc., 135, 11193-11204.
    View publication on PubMed
Search for more literature for 5.4.3.6

Cloned(Commentary)

Cloned (Comment) Organism
gene cctam, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Chondromyces crocatus

Organism

Organism UniProt Comment Textmining
Chondromyces crocatus
-
 gene cctam
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Chondromyces crocatus

Reaction

Reaction Comment Organism Reaction ID
L-tyrosine = 3-amino-3-(4-hydroxyphenyl)propanoate pH-dependent stereochemic mechanism, detailed overview. The enzyme uses a retention-of-configuration mechanism at the amino migration terminus Chondromyces crocatus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine the enzyme isomerizes (S)-alpha-tyrosine to (R)-beta-tyrosine and is stereoselective for (R)-beta-tyrosine (85%), but also forms the (S)-beta-tyrosine enantiomer (15%) through inversion of configuration at both migration termini Chondromyces crocatus 3-amino-3-(4-hydroxyphenyl)propanoate
-
 r
[3-deutero]-(2S,3R)-alpha-tyrosine
-
 Chondromyces crocatus [3-deutero]-(2S,3S)-4'-O,2-N-di((S)-2-methylbutanoic acid)-alpha-tyrosine methyl ester + [3-deutero]-(2S,3R)-4'-O,2-N-di((S)-2-methylbutanoic acid)-alpha-tyrosine methyl ester
-
 ?
[3-deutero]-(2S,3S)-alpha-tyrosine
-
 Chondromyces crocatus [3-deutero]-(2S,3S)-4'-O,2-N-di((S)-2-methylbutanoic acid)-alpha-tyrosine methyl ester + ([3-deutero]-(2S,3R)-4'-O,2-N-di((S)-2-methylbutanoic acid)-alpha-tyrosine methyl ester
-
 ?

Synonyms

Synonyms Comment Organism
TAM
-
 Chondromyces crocatus
tyrosine aminomutase
-
 Chondromyces crocatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31
-
 assay at Chondromyces crocatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Chondromyces crocatus

General Information

General Information Comment Organism
additional information cryptic stereochemistry of the enzyme mechanism, structure-function relationship, detailed overview Chondromyces crocatus