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Literature summary for 5.4.3.6 extracted from
- Discovery of additional members of the tyrosine aminomutase enzyme family and the mutational analysis of CmdF (2009), ChemBioChem, 10, 741-750.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into a pGEX-derived plasmid for expression in Escherichia coli BL21DE3 cells | Chondromyces crocatus |
| into the pCR2.1Topo and pJET1/blunt vector and subsequently into pGEX-6P-1 for expression in Escherichia coli BL21DE3 cells | Myxococcus fulvus Mx f65 |
| into the pJET1/blunt vector and subsequently into pGEX-6P-1 for expression in Escherichia coli BL21DE3 cells | Myxococcus sp. Mx-B0 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C396S | single amino acid alteration | Chondromyces crocatus |
| CmdF_DEL | position 275-288 deleted | Chondromyces crocatus |
| CmdF_MIAQVTSA | position 399-406: EGGQYLAT substituted | Chondromyces crocatus |
| CmdF_MIYMLV | position 62-67: LVPVMI substituted | Chondromyces crocatus |
| CmdF_SAGREDH | position 427-433: NGSNQDV, substituted | Chondromyces crocatus |
| CmdF_SANQEDH | position 427-433: NGSNQDV, substituted | Chondromyces crocatus |
| CmdF_TFLSA | position 81-85: RSHAA, substituted | Chondromyces crocatus |
| CmdF_YHLAT | position 81-85: RSHAA, substituted | Chondromyces crocatus |
| F59Y | single amino acid alteration | Chondromyces crocatus |
| G184R | single amino acid alteration | Chondromyces crocatus |
| K242R | single amino acid alteration | Chondromyces crocatus |
| P377R | single amino acid alteration | Chondromyces crocatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.178 | - |
L-tyrosine | mutant CmdF_MIYMLV | Chondromyces crocatus |  |
| 0.377 | - |
L-tyrosine | wild-type enzme | Chondromyces crocatus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine | Chondromyces crocatus | - |
(R)-beta-tyrosine | - |
? | |
| L-tyrosine | Myxococcus fulvus Mx f65 | - |
(R)-beta-tyrosine | - |
? | |
| L-tyrosine | Myxococcus sp. Mx-B0 | - |
(R)-beta-tyrosine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chondromyces crocatus | Q0VZ68 | - |
- |
| Myxococcus fulvus Mx f65 | B8ZV93 | - |
- |
| Myxococcus sp. Mx-B0 | B8ZV94 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification is carried out by affinity chromatography with GST-Sepharose, the GST-tag is cleaved by using the PreScission protease | Chondromyces crocatus |
| purification is carried out by affinity chromatography with GST-Sepharose, the GST-tag is cleaved by using the PreScission protease | Myxococcus fulvus Mx f65 |
| purification is carried out by affinity chromatography with GST-Sepharose, the GST-tag is cleaved by using the PreScission protease | Myxococcus sp. Mx-B0 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine | - |
Chondromyces crocatus | (R)-beta-tyrosine | - |
? | |
| L-tyrosine | - |
Myxococcus fulvus Mx f65 | (R)-beta-tyrosine | - |
? | |
| L-tyrosine | - |
Myxococcus sp. Mx-B0 | (R)-beta-tyrosine | - |
? | |
| L-tyrosine | - |
Chondromyces crocatus | 4-hydroxycinnamic acid + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CmdF | - |
Chondromyces crocatus |
| MfTAM | - |
Myxococcus fulvus Mx f65 |
| MxTAM | - |
Myxococcus sp. Mx-B0 |
| TAM | - |
Chondromyces crocatus |
| tyrosine aminomutase | - |
Chondromyces crocatus |
| tyrosine aminomutase | - |
Myxococcus fulvus Mx f65 |
| tyrosine aminomutase | - |
Myxococcus sp. Mx-B0 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Chondromyces crocatus |
| 30 | - |
activity assay | Myxococcus fulvus Mx f65 |
| 30 | - |
activity assay | Myxococcus sp. Mx-B0 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
L-tyrosine | mutant CmdF_MIYMLV, product (R)-beta-tyrosine | Chondromyces crocatus | |
| 0.0005 | - |
L-tyrosine | wild-type enzme, product para-hydroxycinnamic acid | Chondromyces crocatus | |
| 0.0013 | - |
L-tyrosine | mutant CmdF_MIYMLV, product para-hydroxycinnamic acid | Chondromyces crocatus | |
| 0.0072 | - |
L-tyrosine | wild-type enzme, product (R)-beta-tyrosine | Chondromyces crocatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
activity assay | Chondromyces crocatus |
| 8.8 | - |
activity assay | Myxococcus fulvus Mx f65 |
| 8.8 | - |
activity assay | Myxococcus sp. Mx-B0 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | (R)-beta-tyrosine is incorporated into the chondramides produced by Chondromyces crocatus | Chondromyces crocatus |
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