Cloned (Comment) | Organism |
---|---|
into a pGEX-derived plasmid for expression in Escherichia coli BL21DE3 cells | Chondromyces crocatus |
into the pCR2.1Topo and pJET1/blunt vector and subsequently into pGEX-6P-1 for expression in Escherichia coli BL21DE3 cells | Myxococcus fulvus Mx f65 |
into the pJET1/blunt vector and subsequently into pGEX-6P-1 for expression in Escherichia coli BL21DE3 cells | Myxococcus sp. Mx-B0 |
Protein Variants | Comment | Organism |
---|---|---|
C396S | single amino acid alteration | Chondromyces crocatus |
CmdF_DEL | position 275-288 deleted | Chondromyces crocatus |
CmdF_MIAQVTSA | position 399-406: EGGQYLAT substituted | Chondromyces crocatus |
CmdF_MIYMLV | position 62-67: LVPVMI substituted | Chondromyces crocatus |
CmdF_SAGREDH | position 427-433: NGSNQDV, substituted | Chondromyces crocatus |
CmdF_SANQEDH | position 427-433: NGSNQDV, substituted | Chondromyces crocatus |
CmdF_TFLSA | position 81-85: RSHAA, substituted | Chondromyces crocatus |
CmdF_YHLAT | position 81-85: RSHAA, substituted | Chondromyces crocatus |
F59Y | single amino acid alteration | Chondromyces crocatus |
G184R | single amino acid alteration | Chondromyces crocatus |
K242R | single amino acid alteration | Chondromyces crocatus |
P377R | single amino acid alteration | Chondromyces crocatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.178 | - |
L-tyrosine | mutant CmdF_MIYMLV | Chondromyces crocatus | |
0.377 | - |
L-tyrosine | wild-type enzme | Chondromyces crocatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine | Chondromyces crocatus | - |
(R)-beta-tyrosine | - |
? | |
L-tyrosine | Myxococcus fulvus Mx f65 | - |
(R)-beta-tyrosine | - |
? | |
L-tyrosine | Myxococcus sp. Mx-B0 | - |
(R)-beta-tyrosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chondromyces crocatus | Q0VZ68 | - |
- |
Myxococcus fulvus Mx f65 | B8ZV93 | - |
- |
Myxococcus sp. Mx-B0 | B8ZV94 | - |
- |
Purification (Comment) | Organism |
---|---|
purification is carried out by affinity chromatography with GST-Sepharose, the GST-tag is cleaved by using the PreScission protease | Chondromyces crocatus |
purification is carried out by affinity chromatography with GST-Sepharose, the GST-tag is cleaved by using the PreScission protease | Myxococcus fulvus Mx f65 |
purification is carried out by affinity chromatography with GST-Sepharose, the GST-tag is cleaved by using the PreScission protease | Myxococcus sp. Mx-B0 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine | - |
Chondromyces crocatus | (R)-beta-tyrosine | - |
? | |
L-tyrosine | - |
Myxococcus fulvus Mx f65 | (R)-beta-tyrosine | - |
? | |
L-tyrosine | - |
Myxococcus sp. Mx-B0 | (R)-beta-tyrosine | - |
? | |
L-tyrosine | - |
Chondromyces crocatus | 4-hydroxycinnamic acid + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CmdF | - |
Chondromyces crocatus |
MfTAM | - |
Myxococcus fulvus Mx f65 |
MxTAM | - |
Myxococcus sp. Mx-B0 |
TAM | - |
Chondromyces crocatus |
tyrosine aminomutase | - |
Chondromyces crocatus |
tyrosine aminomutase | - |
Myxococcus fulvus Mx f65 |
tyrosine aminomutase | - |
Myxococcus sp. Mx-B0 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Chondromyces crocatus |
30 | - |
activity assay | Myxococcus fulvus Mx f65 |
30 | - |
activity assay | Myxococcus sp. Mx-B0 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
L-tyrosine | mutant CmdF_MIYMLV, product (R)-beta-tyrosine | Chondromyces crocatus | |
0.0005 | - |
L-tyrosine | wild-type enzme, product para-hydroxycinnamic acid | Chondromyces crocatus | |
0.0013 | - |
L-tyrosine | mutant CmdF_MIYMLV, product para-hydroxycinnamic acid | Chondromyces crocatus | |
0.0072 | - |
L-tyrosine | wild-type enzme, product (R)-beta-tyrosine | Chondromyces crocatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
activity assay | Chondromyces crocatus |
8.8 | - |
activity assay | Myxococcus fulvus Mx f65 |
8.8 | - |
activity assay | Myxococcus sp. Mx-B0 |
General Information | Comment | Organism |
---|---|---|
metabolism | (R)-beta-tyrosine is incorporated into the chondramides produced by Chondromyces crocatus | Chondromyces crocatus |