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Literature summary for 5.4.3.6 extracted from
- Biosynthesis of beta-tyrosine (1972), Biochim. Biophys. Acta, 264, 1-10.
General Stability
| General Stability | Organism |
|---|---|
| stable to freezing and thawing | Brevibacillus brevis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Brevibacillus brevis |  |
| NaBH4 | - |
Brevibacillus brevis | |
| p-hydroxycinnamate | - |
Brevibacillus brevis | |
| p-hydroxyphenylpyruvate | - |
Brevibacillus brevis | |
| phenylhydrazine | - |
Brevibacillus brevis | |
| phenylpyruvate | - |
Brevibacillus brevis | |
| pyruvate | - |
Brevibacillus brevis | |
| Semicarbazide | - |
Brevibacillus brevis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Brevibacillus brevis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Tyr | Brevibacillus brevis | formation of beta-Tyr which is the N-terminal amino acid of the antibiotic edeine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacillus brevis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brevibacillus brevis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00188 | - |
- |
Brevibacillus brevis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 3 months | Brevibacillus brevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Tyr | - |
Brevibacillus brevis | 3-Amino-3-(4-hydroxyphenyl)propanoate | i.e. beta-Tyr | ? | |
| L-Tyr | formation of beta-Tyr which is the N-terminal amino acid of the antibiotic edeine | Brevibacillus brevis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Brevibacillus brevis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | 39 | active in this range | Brevibacillus brevis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
75% loss of activity after 24 h at room temperature | Brevibacillus brevis |
| 60 | - |
5 min, complete inactivation | Brevibacillus brevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Brevibacillus brevis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | about 10% of maximal activity at pH 8 and 9 | Brevibacillus brevis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | required | Brevibacillus brevis | |
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