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Literature summary for 5.4.3.3 extracted from
- Purification and properties of beta-lysine mutase, a pyridoxal phosphate and B12 coenzyme dependent enzyme (1973), Biochemistry, 12, 1054-1063.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2'-deoxyATP | stimulates | Acetoanaerobium sticklandii |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Diethanolamine/HCl | - |
Acetoanaerobium sticklandii | |
| hydrazine | - |
Acetoanaerobium sticklandii | |
| Hydroxyadenylcobamide | formed during isolation by degradation of enzyme-bound cobamide, strong inhibitor, tightly bound to the protein. Incubation with cobalamin, Mg2+, a mercaptan, and pyridoxal 5'-phosphate displaces the hydroxyadenylcobamide and markedly activates the enzyme | Acetoanaerobium sticklandii | |
| hydroxylamine | - |
Acetoanaerobium sticklandii | |
| Isonicotinic acid hydrazide | - |
Acetoanaerobium sticklandii | |
| additional information | during catalysis under conditions where the mutase product 3,5-diaminohexanoate is not continuously removed there is extensive cleavage of cobamamide coenzyme to free 5'-deoxyadenosine and cobalamin with concomitant inactivation of the enzyme. This inactivation is prevented by the addition of sulfhydryl protein and ATP, oxygen accelerates inactivation | Acetoanaerobium sticklandii | |
| NaBH4 | - |
Acetoanaerobium sticklandii | |
| phenylhydrazine | - |
Acetoanaerobium sticklandii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | monovalent cation, e.g. K+ required | Acetoanaerobium sticklandii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000 + 2 * 52000, SDS-PAGE | Acetoanaerobium sticklandii |
| 52000 | - |
2 * 32000 + 2 * 52000, SDS-PAGE | Acetoanaerobium sticklandii |
| 170000 | - |
gel filtration | Acetoanaerobium sticklandii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetoanaerobium sticklandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetoanaerobium sticklandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.5 | - |
unit defined as the amounts which allows the formation of 0.001 mM NADPH per min in a coupled assay system | Acetoanaerobium sticklandii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -22°C, stable for at least 3 months. After 6 months, with repeated freezing and thawing, two-third of the original activity remains | Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,6-Diaminohexanoate | - |
Acetoanaerobium sticklandii | 3,5-Diaminohexanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 32000 + 2 * 52000, SDS-PAGE | Acetoanaerobium sticklandii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
highest activity in triethanolamine/HCl buffer | Acetoanaerobium sticklandii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Cobalamin | dependent on | Acetoanaerobium sticklandii | |
| pyridoxal 5'-phosphate | absolutely dependent on, Km: 0.00022 mM | Acetoanaerobium sticklandii | |
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