Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyATP | stimulates | Acetoanaerobium sticklandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diethanolamine/HCl | - |
Acetoanaerobium sticklandii | |
hydrazine | - |
Acetoanaerobium sticklandii | |
Hydroxyadenylcobamide | formed during isolation by degradation of enzyme-bound cobamide, strong inhibitor, tightly bound to the protein. Incubation with cobalamin, Mg2+, a mercaptan, and pyridoxal 5'-phosphate displaces the hydroxyadenylcobamide and markedly activates the enzyme | Acetoanaerobium sticklandii | |
hydroxylamine | - |
Acetoanaerobium sticklandii | |
Isonicotinic acid hydrazide | - |
Acetoanaerobium sticklandii | |
additional information | during catalysis under conditions where the mutase product 3,5-diaminohexanoate is not continuously removed there is extensive cleavage of cobamamide coenzyme to free 5'-deoxyadenosine and cobalamin with concomitant inactivation of the enzyme. This inactivation is prevented by the addition of sulfhydryl protein and ATP, oxygen accelerates inactivation | Acetoanaerobium sticklandii | |
NaBH4 | - |
Acetoanaerobium sticklandii | |
phenylhydrazine | - |
Acetoanaerobium sticklandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation, e.g. K+ required | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000 + 2 * 52000, SDS-PAGE | Acetoanaerobium sticklandii |
52000 | - |
2 * 32000 + 2 * 52000, SDS-PAGE | Acetoanaerobium sticklandii |
170000 | - |
gel filtration | Acetoanaerobium sticklandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetoanaerobium sticklandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.5 | - |
unit defined as the amounts which allows the formation of 0.001 mM NADPH per min in a coupled assay system | Acetoanaerobium sticklandii |
Storage Stability | Organism |
---|---|
-22°C, stable for at least 3 months. After 6 months, with repeated freezing and thawing, two-third of the original activity remains | Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,6-Diaminohexanoate | - |
Acetoanaerobium sticklandii | 3,5-Diaminohexanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 32000 + 2 * 52000, SDS-PAGE | Acetoanaerobium sticklandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
highest activity in triethanolamine/HCl buffer | Acetoanaerobium sticklandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | dependent on | Acetoanaerobium sticklandii | |
pyridoxal 5'-phosphate | absolutely dependent on, Km: 0.00022 mM | Acetoanaerobium sticklandii |