Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.3.2 extracted from

  • Chen, D.; Frey, P.A.; Lepore, B.W.; Ringe, D.; Ruzicka, F.J.
    Identification of structural and catalytic classes of highly conserved amino acid residues in lysine 2,3-aminomutase (2006), Biochemistry, 45, 12647-12653.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D165N about 0.1% of wild-type activity, 0.23 mol iron and 0.13 mol sulfur per mol of subunit Clostridium subterminale
D172N no catalytic activity, 0.43 mol iron and 0.13 mol sulfur per mol of subunit Clostridium subterminale
D293N no catalytic activity, 0.97 mol iron and 0.05 mol sulfur per mol of subunit. Involved in binding of epsilon-aminium and alpha-carboxylate groups of substrate Clostridium subterminale
D330A no catalytic activity, 1.9 mol iron and 1.3 mol sulfur per mol of subunit. Involved in binding of epsilon-aminium and alpha-carboxylate groups of substrate Clostridium subterminale
D330N no catalytic activity, 1.0 mol iron and 0.63 mol sulfur per mol of subunit Clostridium subterminale
D96N less than 1% of wild-type activity, 1.2 mol iron and 0.95 mol sulfur per mol of subunit Clostridium subterminale
E236Q about 1% of wild-type activity, 0.7 mol iron and 0.5 mol sulfur per mol of subunit Clostridium subterminale
E86Q about 20% of wild-type activity, 0.5 mol iron and 0.4 mol sulfur per mol of subunit Clostridium subterminale
R130K no catalytic activity, 0.2 mol iron and 0.3 mol sulfur per mol of subunit Clostridium subterminale
R130Q no catalytic activity, 0.42 mol iron and 0.2 mol sulfur per mol of subunit Clostridium subterminale
R134K no catalytic activity, 1.5 mol iron and 1.5 mol sulfur per mol of subunit Clostridium subterminale
R134Q no catalytic activity, 2.8 mol iron and 2.7 mol sulfur per mol of subunit. Involved in binding of epsilon-aminium and alpha-carboxylate groups of substrate Clostridium subterminale
R135K about 16% of wild-type activity, 2.4 mol iron and 1.6 mol sulfur per mol of subunit Clostridium subterminale
R135Q about 1.5% of wild-type activity, 1.8 mol iron and 1.5 mol sulfur per mol of subunit Clostridium subterminale
R136Q about 1.2% of wild-type activity, 0.48 mol iron and 0.35 mol sulfur per mol of subunit Clostridium subterminale

Metals/Ions

Metals/Ions Comment Organism Structure
Iron [4Fe-4S] cluster, 2.7 mol of iron per mol of subunit Clostridium subterminale

Organism

Organism UniProt Comment Textmining
Clostridium subterminale Q9XBQ8
-
-
Clostridium subterminale SB4 Q9XBQ8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62
-
recombinant wild-type with His-tag Clostridium subterminale
64
-
wild-type Clostridium subterminale
88
-
wild-type, Fe-S centers reconstituted Clostridium subterminale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine
-
Clostridium subterminale (3S)-3,6-diaminohexanoate
-
?
L-lysine
-
Clostridium subterminale SB4 (3S)-3,6-diaminohexanoate
-
?