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Literature summary for 5.4.3.2 extracted from

  • Chirpich, T.P.; Zappia, V.; Costilow, R.N.; Barker, H.A.
    Lysine 2,3-aminomutase. Purification and properties of a pyridoxal phosphate and S-adenosylmethionine-activated enzyme (1970), J. Biol. Chem., 245, 1778-1789.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
S-adenosylmethionine activates Clostridium sp.
S-adenosylmethionine maximal activity with 3.7 M per mol of enzyme Clostridium sp.
S-adenosylmethionine Km: 0.000028 mM Clostridium sp.
sulfhydryl compound can reactivate oxygen-inactivated enzyme, optimal activation when 0.9 mM Fe2+ is present Clostridium sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.1 mM, 80% inhibition Clostridium sp.
S-adenosylhomocysteine competitive towards S-adenosylmethionine Clostridium sp.
S-aminoethylcysteine
-
 Clostridium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.6
-
 L-Lys
-
 Clostridium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ sulfhydryl compound + 0.9 mM Fe2+ reactivates oxygen-inactivated enzyme Clostridium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
285000
-
 gel filtration, disc gel electrophoresis Clostridium sp.

Organism

Organism UniProt Comment Textmining
Clostridium sp.
-
-
-
Clostridium sp. SB4
-
-
-

Oxidation Stability

Oxidation Stability Organism
rapid inactivation by oxygen Clostridium sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
-
 Clostridium sp.

Storage Stability

Storage Stability Organism
-10°C, under argon, 30 mM Tris/Cl buffer, pH 7.8, 0.01 mM pyridoxal phosphate, 15% v/v glycerol, 10-15% loss of activity after 3 months Clostridium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Lys r Clostridium sp. (3S)-3,6-diaminohexanoic acid
-
 ?
L-Lys reaction with D-Lys is less than 1% of the reaction with L-Lys Clostridium sp. (3S)-3,6-diaminohexanoic acid
-
 ?
L-Lys r Clostridium sp. SB4 (3S)-3,6-diaminohexanoic acid
-
 ?
L-Lys reaction with D-Lys is less than 1% of the reaction with L-Lys Clostridium sp. SB4 (3S)-3,6-diaminohexanoic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Clostridium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 about 25% of maximal activity at 10°C and 60°C Clostridium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Clostridium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9.8 about 50% of maximal activity at pH 6.0 and 9.8 Clostridium sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate activates Clostridium sp.