Literature summary for 5.4.2.8 extracted from

Yoon, S.; Park, S.; Kim, Y.; Shin, M.; Chong, C.; Choi, J.
Cloning and characterization of phosphoglucomutase and phosphomannomutase derived from Sphingomonas chungbukensis DJ77 (2008), J. Biochem., 144, 507-512.

Search for more literature for 5.4.2.8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Sphingobium chungbukense

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1569	-	alpha-D-mannose 1-phosphate	in 50 mM Tris-HCl (pH 7.5), at 37°C	Sphingobium chungbukense
0.4516	-	alpha-D-glucose 1-phosphate	in 50 mM Tris-HCl (pH 7.5), at 37°C	Sphingobium chungbukense

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	highest activity in the presence of Mg2+	Sphingobium chungbukense

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51000	-	2 * 51000, SDS-PAGE	Sphingobium chungbukense
105400	-	native enzyme, non-denaturing PAGE	Sphingobium chungbukense

Organism

Organism	UniProt	Comment	Textmining
Sphingobium chungbukense	-	-	-
Sphingobium chungbukense DJ77	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-chelating resin chromatography	Sphingobium chungbukense

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucose 1-phosphate	-	Sphingobium chungbukense	alpha-D-glucose 6-phosphate	-	?
alpha-D-glucose 1-phosphate	-	Sphingobium chungbukense DJ77	alpha-D-glucose 6-phosphate	-	?
alpha-D-mannose 1-phosphate	preferred substrate	Sphingobium chungbukense	alpha-D-mannose 6-phosphate	-	?
alpha-D-mannose 1-phosphate	preferred substrate	Sphingobium chungbukense DJ77	alpha-D-mannose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 51000, SDS-PAGE	Sphingobium chungbukense

Synonyms

Synonyms	Comment	Organism
PGM/PMM	-	Sphingobium chungbukense
SP-2	bifunctional enzyme with phosphoglucomutase and phosphomannomutase activities	Sphingobium chungbukense

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
82.93	-	alpha-D-glucose 1-phosphate	in 50 mM Tris-HCl (pH 7.5), at 37°C	Sphingobium chungbukense
134.9	-	alpha-D-mannose 1-phosphate	in 50 mM Tris-HCl (pH 7.5), at 37°C	Sphingobium chungbukense

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Sphingobium chungbukense

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9	-	Sphingobium chungbukense

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Release 2023.1 (January 2023)