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Literature summary for 5.4.2.8 extracted from

  • Akutsu, J.; Zhang, Z.; Tsujimura, M.; Sasaki, M.; Yohda, M.; Kawarabayasi, Y.
    Characterization of a thermostable enzyme with phosphomannomutase/phosphoglucomutase activities from the hyperthermophilic archaeon Pyrococcus horikoshii OT3 (2005), J. Biochem., 138, 159-166.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
S101A mutant protein PH0923S101A to investigate the role of the serine residue Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KM mannose-1-phosphate isoenzyme PH0923S101A not detectable, 37°C Pyrococcus horikoshii
0.0915
-
D-mannose-1-phosphate isoenzyme PH0923, 37°C Pyrococcus horikoshii
0.0952
-
D-glucose-1-phosphate isoenzyme PH0923, 65°C Pyrococcus horikoshii
0.127
-
D-glucose-1-phosphate isoenzyme PH0923S101A, 65°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1% relative activity Pyrococcus horikoshii
Co2+ 44% relative activity Pyrococcus horikoshii
Mg2+ 100% relative activity Pyrococcus horikoshii
Mn2+ 20% relative activity Pyrococcus horikoshii
additional information no detectable activity after addition of 0.1 mM EDTA Pyrococcus horikoshii
Ni2+ 15% relative activity Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
OT3
-
Pyrococcus horikoshii OT-3
-
OT3
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity column chromatography Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
isoenzyme PH0923 PGM Vmax 24, 65°C Pyrococcus horikoshii
additional information
-
isoenzyme PH0923 PMM Vmax 0.7, 37°C Pyrococcus horikoshii
additional information
-
isoenzyme PH0923S101A PGM Vmax 0.4, 65°C Pyrococcus horikoshii
additional information
-
isoenzyme PH0923S101A PMM Vmax not detectable, 37°C Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose-1-phosphate
-
Pyrococcus horikoshii D-glucose-6-phosphate
-
r
D-glucose-1-phosphate
-
Pyrococcus horikoshii OT-3 D-glucose-6-phosphate
-
r
D-mannose-1-phosphate
-
Pyrococcus horikoshii D-mannose-6-phosphate
-
r
D-mannose-1-phosphate
-
Pyrococcus horikoshii OT-3 D-mannose-6-phosphate
-
r

Synonyms

Synonyms Comment Organism
PMM/PGM bifunctional enzyme Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
longer than 90 min stable Pyrococcus horikoshii
100
-
50% activity after 5 min Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10
-
Pyrococcus horikoshii