BRENDA - Enzyme Database show
show all sequences of 5.4.2.7

A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima

Moustafa, H.M.; Zaghloul, T.I.; Zhang, Y.H.; Anal. Biochem. 501, 75-81 (2016)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
100% activity at 0.5 mM
Thermotoga maritima
alpha-D-glucose1,6-bisphosphate
-
Thermotoga maritima
dithiothreitol
100% activity at 0.5 mM
Thermotoga maritima
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) cells
Thermotoga maritima
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.75
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 60C
Thermotoga maritima
1.2
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 90C
Thermotoga maritima
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
required, 100% activity at 0.1 mM
Thermotoga maritima
additional information
the presence of Ni2+, Co2+, Zn2+, and Cu2+ enhances enzyme activities, but not as much as Mn2+
Thermotoga maritima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-D-ribose 1-phosphate
Thermotoga maritima
-
alpha-D-ribose 5-phosphate
-
-
r
alpha-D-ribose 5-phosphate
Thermotoga maritima
-
alpha-D-ribose 1-phosphate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermotoga maritima
Q9WY14
-
-
Purification (Commentary)
Commentary
Organism
IMAC Ni-charged resin column chromatography, and Superdex 200 gel filtration
Thermotoga maritima
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
14
-
crude enzyme, at pH 7.5 and 90C
Thermotoga maritima
57.8
-
after 4.1fold purification, at pH 7.5 and 90C
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-D-ribose 1-phosphate
-
746733
Thermotoga maritima
alpha-D-ribose 5-phosphate
-
-
-
r
alpha-D-ribose 5-phosphate
-
746733
Thermotoga maritima
alpha-D-ribose 1-phosphate
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 42000, SDS-PAGE
Thermotoga maritima
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
90
-
-
Thermotoga maritima
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
100
about 30% activity at 30C, about 40% activity at 40C, about 50% activity at 50C, about 60% activity at 60C, about 70% activity at 70C, about 80% activity at 80C, 100% activity at 90C, about 90% activity at 100C
Thermotoga maritima
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
55.6
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 60C
Thermotoga maritima
185
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 90C
Thermotoga maritima
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
in 100 mM MOPS buffer
Thermotoga maritima
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8
about 40% activity at pH 6.5, more than 70% activity between pH 7.0 and 8.0
Thermotoga maritima
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
100% activity at 0.5 mM
Thermotoga maritima
alpha-D-glucose1,6-bisphosphate
-
Thermotoga maritima
dithiothreitol
100% activity at 0.5 mM
Thermotoga maritima
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) cells
Thermotoga maritima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.75
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 60C
Thermotoga maritima
1.2
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 90C
Thermotoga maritima
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
required, 100% activity at 0.1 mM
Thermotoga maritima
additional information
the presence of Ni2+, Co2+, Zn2+, and Cu2+ enhances enzyme activities, but not as much as Mn2+
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-D-ribose 1-phosphate
Thermotoga maritima
-
alpha-D-ribose 5-phosphate
-
-
r
alpha-D-ribose 5-phosphate
Thermotoga maritima
-
alpha-D-ribose 1-phosphate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
IMAC Ni-charged resin column chromatography, and Superdex 200 gel filtration
Thermotoga maritima
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
14
-
crude enzyme, at pH 7.5 and 90C
Thermotoga maritima
57.8
-
after 4.1fold purification, at pH 7.5 and 90C
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-D-ribose 1-phosphate
-
746733
Thermotoga maritima
alpha-D-ribose 5-phosphate
-
-
-
r
alpha-D-ribose 5-phosphate
-
746733
Thermotoga maritima
alpha-D-ribose 1-phosphate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42000, SDS-PAGE
Thermotoga maritima
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
90
-
-
Thermotoga maritima
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
100
about 30% activity at 30C, about 40% activity at 40C, about 50% activity at 50C, about 60% activity at 60C, about 70% activity at 70C, about 80% activity at 80C, 100% activity at 90C, about 90% activity at 100C
Thermotoga maritima
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
55.6
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 60C
Thermotoga maritima
185
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 90C
Thermotoga maritima
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
in 100 mM MOPS buffer
Thermotoga maritima
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8
about 40% activity at pH 6.5, more than 70% activity between pH 7.0 and 8.0
Thermotoga maritima
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
74.1
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 60C
Thermotoga maritima
154
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 90C
Thermotoga maritima
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
74.1
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 60C
Thermotoga maritima
154
-
alpha-D-ribose 5-phosphate
at pH 7.5 and 90C
Thermotoga maritima
Other publictions for EC 5.4.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748749
Meyer
Methanol-essential growth of ...
Escherichia coli, Escherichia coli MeSV1
Nat. Commun.
9
1508
2018
-
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-
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2
-
3
-
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2
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2
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748271
Rivero
Biosynthesis of an antiviral ...
Escherichia coli, Escherichia coli ATCC 4157
J. Biotechnol.
249
34-41
2017
1
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1
-
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1
-
-
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1
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2
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6
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1
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2
1
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1
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1
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1
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1
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1
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1
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2
1
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1
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746733
Moustafa
A simple assay for determinin ...
Thermotoga maritima
Anal. Biochem.
501
75-81
2016
3
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1
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2
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2
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2
-
4
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1
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2
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2
1
1
1
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2
1
1
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1
1
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2
1
1
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2
2
726977
Iverson
Molecular differences between ...
Bacillus cereus, Bacillus cereus DSM 31
Biochemistry
51
1964-1975
2012
1
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1
1
4
1
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1
1
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1
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1
1
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727521
Walther
The PGM3 gene encodes the majo ...
Saccharomyces cerevisiae
FEBS Lett.
586
4114-4118
2012
1
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1
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2
-
1
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2
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2
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1
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3
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1
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1
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1
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1
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3
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3
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1
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1
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1
1
-
-
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715660
Panosian
Bacillus cereus phosphopentomu ...
Bacillus cereus, Bacillus cereus ATCC 14579
J. Biol. Chem.
286
8043-8054
2011
1
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1
1
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1
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2
1
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3
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1
1
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1
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1
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1
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1
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713648
Panosian
Crystallization and preliminar ...
Bacillus cereus, Bacillus cereus ATCC 14579D
Acta Crystallogr. Sect. F
66
811-814
2010
-
-
1
1
-
-
-
-
-
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1
-
-
4
-
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1
-
-
-
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2
1
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1
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1
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1
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705839
Horinouchi
Screening and characterization ...
Escherichia coli, Lysinibacillus sphaericus, Lysinibacillus sphaericus AKU 229
N. Biotechnol.
26
75-82
2009
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1
2
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6
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695174
Taverna-Porro
-
Chemoenzymic preparation of nu ...
Escherichia coli
Tetrahedron Lett.
49
2642-2645
2008
1
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1
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662012
Rashid
Presence of a novel phosphopen ...
Thermococcus kodakarensis
J. Bacteriol.
186
4185-4191
2004
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1
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1
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1
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1
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1
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3348
Hamamoto
Phosphopentomutase of Bacillus ...
Escherichia coli, Geobacillus stearothermophilus, Geobacillus stearothermophilus TH6-2
Biosci. Biotechnol. Biochem.
62
1103-1108
1998
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1
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3
3
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5
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1
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1
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18
2
2
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4
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1
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1
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3
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18
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2
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4
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1
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1
1
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3347
Barbas
-
Overexpression and substrate s ...
Escherichia coli
Bioorg. Chem.
19
261-269
1991
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3345
Ipata
Induction of phosphoribomutase ...
Bacillus cereus
Biochim. Biophys. Acta
755
253-256
1983
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3346
Barsky
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
743
162-171
1983
2
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1
1
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2
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3344
Tozzi
A coupled optical enzyme assay ...
Escherichia coli
Anal. Biochem.
123
265-269
1982
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3343
Leer
Multiple forms of phosphodeoxy ...
Escherichia coli
Biochemistry
14
599-607
1975
1
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3342
Nygaard
Nucleoside-catabolizing enzyme ...
Salmonella enterica subsp. enterica serovar Typhimurium
Eur. J. Biochem.
36
267-272
1973
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1
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1
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1
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3
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7
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3
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1
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1
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1
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2
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1
1
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1
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1
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1
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