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Literature summary for 5.4.2.7 extracted from

  • Panosian, T.D.; Nannemann, D.P.; Watkins, G.R.; Phelan, V.V.; McDonald, W.H.; Wadzinski, B.E.; Bachmann, B.O.; Iverson, T.M.
    Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle (2011), J. Biol. Chem., 286, 8043-8054.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
D-Glucose 1,6-bisphosphate there is a concentration-dependent enhancement of PPM activity between 0.0001 mM and 0.0025 mM D-glucose 1,6-bisphosphate Bacillus cereus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Gold (DE3) cells Bacillus cereus

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme alone, co-crystallized with ribose 5-phosphate, co-crystallized with glucose 1,6-bisphosphate, and following activation with glucose 1,6-bisphosphate, hanging drop vapor diffusion method, using 100 mM Bis-Tris, pH 5.5, 50 mM MnCl2, 14% (w/v) polyethylene glycol 3350, and 75 mM NH4CH3COO Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.263
-
D-ribose 5-phosphate in 25 mM Tris-HCl, pH 8.0, and 0.1 mM MnCl2, 0.0005 mM glucose 1,6-bisphosphate,at 23°C Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ only Mn2+-incorporated enzyme shows significant enzyme activity Bacillus cereus
additional information not dependent on Zn2+, Ni2+, Mg2+, and Co2+ Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, SDS-PAGE Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus Q818Z9
-
-
Bacillus cereus ATCC 14579 Q818Z9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein protein phosphorylation activates the enzyme Bacillus cereus

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-ribose 1-phosphate
-
Bacillus cereus D-ribose 5-phosphate
-
r
alpha-D-ribose 1-phosphate
-
Bacillus cereus ATCC 14579 D-ribose 5-phosphate
-
r

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Bacillus cereus

Synonyms

Synonyms Comment Organism
PPM
-
Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.2
-
D-ribose 5-phosphate in 25 mM Tris-HCl, pH 8.0, and 0.1 mM MnCl2, 0.0005 mM glucose 1,6-bisphosphate,at 23°C Bacillus cereus