Cloned (Comment) | Organism |
---|---|
gene nst, DNA and amino acid sequence determination, gene nst is allelic to CG10627, expression analysis | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Drosophila melanogaster | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine 6-phosphate | Drosophila melanogaster | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VTZ4 | gene nst | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
fibroblast | - |
Drosophila melanogaster | - |
mesoderm | - |
Drosophila melanogaster | - |
trachea | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine 6-phosphate | - |
Drosophila melanogaster | N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PGM3 | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
malfunction | loss-of-function mutations in the nst gene, which encodes PGM3, block fibroblast growth factor signaling during embryonic mesoderm and tracheal development. In nst mutants with an 80% reduction in the amount of UDP-GlcNAc, O-GlcNAcylation is compromised. Embryos maternally and zygotically mutant for nst exhibit defects in mesoderm layer formation during gastrulation that are similar to those observed in embryos with mutations in the gene encoding the fibroblast growth factor receptor Heartless (Htl). Zygotic nst mutants (nstZ) exhibit normal dorsal mesoderm differentiation, embryos homozygous for the hypomorphic htlYY262 allele show a reduction in dorsal mesoderm. Phenotypes, overview. Dof is the critical component in MAPK activation affected in nst mutants | Drosophila melanogaster |
metabolism | Nst acts downstream of fibroblast growth factor receptor signaling | Drosophila melanogaster |
physiological function | the enzyme is required for normal cellular levels of UDP-GlcNAc and essential in UDP-GlcNAc biosynthesis, and for fibroblast growth factor signaling in early embryos. Role for Nst in fibroblast growth factor receptor Heartless (Htl) signaling | Drosophila melanogaster |