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Literature summary for 5.4.2.3 extracted from

  • Bandini, G.; Marino, K.; Guether, M.L.; Wernimont, A.K.; Kuettel, S.; Qiu, W.; Afzal, S.; Kelner, A.; Hui, R.; Ferguson, M.A.
    Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase (2012), Mol. Microbiol., 85, 513-534.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.2.3

Cloned(Commentary)

Cloned (Comment) Organism
gene PAGM, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information RNAi knockdown of gene TbPAGM, construction of a cell line expressing tetracycline inducible double-stranded RNA targeting TbPMM, induction of dsRNA targeting TbPMM results in a 68% knockdown of TbPMM mRNA after 48 h and a reduction of growth rate that leads to cell death after 72 h, phenotypes, overview Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 1-phosphate competitive inhibition Trypanosoma brucei
D-glucose 6-phosphate competitive inhibition Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 N-acetyl-D-glucosamine 6-phosphate pH 7.3, 37°C Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Trypanosoma brucei 5829
-
glycosome
-
 Trypanosoma brucei 20015
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma brucei the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview ?
-
 ?
additional information Trypanosoma brucei 427 the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview ?
-
 ?
N-acetyl-alpha-D-glucosamine 1-phosphate Trypanosoma brucei
-
 N-acetyl-D-glucosamine 6-phosphate
-
 r
N-acetyl-alpha-D-glucosamine 1-phosphate Trypanosoma brucei 427
-
 N-acetyl-D-glucosamine 6-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei F4NCC3 gene PAGM
-
Trypanosoma brucei 427 F4NCC3 gene PAGM
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Trypanosoma brucei

Source Tissue

Source Tissue Comment Organism Textmining
additional information quantitative proteomic analysis show that the TbPAGM protein is present in both life cycle stages, procyclic and bloodstream forms Trypanosoma brucei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei ?
-
 ?
additional information the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei 427 ?
-
 ?
N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Trypanosoma brucei N-acetyl-D-glucosamine 6-phosphate
-
 r
N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Trypanosoma brucei 427 N-acetyl-D-glucosamine 6-phosphate
-
 r
N-acetyl-D-glucosamine 6-phosphate
-
 Trypanosoma brucei N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
N-acetyl-D-glucosamine 6-phosphate
-
 Trypanosoma brucei 427 N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Synonyms

Synonyms Comment Organism
PAGM
-
 Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 9.1 broad optimum Trypanosoma brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0063
-
 versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37°C Trypanosoma brucei D-glucose 1-phosphate
0.0083
-
 versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37°C Trypanosoma brucei D-glucose 6-phosphate

General Information

General Information Comment Organism
evolution the enzyme belongs to the alpha-D-phosphohexomutase superfamily of enzymes Trypanosoma brucei
physiological function the enzyme is essential for parasite growth. It takes over the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16
-
 N-acetyl-D-glucosamine 6-phosphate pH 7.3, 37°C Trypanosoma brucei