Activating Compound | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | metal chelator required | Sus scrofa | |
Cys | metal chelator required | Sus scrofa | |
diethyldithiocarbamate | metal chelator required | Sus scrofa | |
fructose 1,6-diphosphate | or glucose 1,6-diphosphate activates, Km: 0.018 mM | Sus scrofa | |
glucose 1,6-diphosphate | or fructose 1,6-diphosphate activates, Km: 0.002 mM | Sus scrofa | |
sulfhydryl compound | increases activity | Sus scrofa |
General Stability | Organism |
---|---|
purified enzyme loses more than 90% activity upon one freezing and thawing | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Be2+ | 0.02 mM in presence of 1 mM Mg2+, complete inhibition | Sus scrofa | |
Cd2+ | 0.5 mM in presence of 1 mM Mg2+, complete inhibition | Sus scrofa | |
Cu2+ | 0.5 mM in presence of 1 mM Mg2+, complete inhibition | Sus scrofa | |
Mn2+ | 1 mM in presence of 1 mM Mg2+, complete inhibition | Sus scrofa | |
Zn2+ | 0.5 mM in presence of 1 mM Mg2+, complete inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
N-acetylglucosamine 1-phosphate | - |
Sus scrofa | |
0.39 | - |
glucose 1-phosphate | - |
Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent metal ion required. Optimal Mg2+ concentration in the presence of dithiothreitol is 10 mM, maximal activity with other metal chelators is obtained with 1 mM Mg2+ | Sus scrofa | |
Mn2+ | divalent metal ion required. Mn2+ is less efficient than Mg2+ | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
submandibular gland | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
0°C, 30% sucrose or 30% glycerol, 50% loss of activity after 3 weeks. Addition of albumin or varying the pH does not appreciably enhance the stability of the enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glucose 1-phosphate | - |
Sus scrofa | Glucose 6-phosphate | - |
? | |
N-Acetyl-D-glucosamine 1-phosphate | - |
Sus scrofa | N-Acetyl-D-glucosamine 6-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | Tris-HCl buffer | Sus scrofa |
7 | 7.5 | Tris-maleate buffer | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 45% of maximal activity, pH 9.0: about 40% of maximal activity, Tris-maleate buffer | Sus scrofa |