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Literature summary for 5.4.2.2 extracted from

  • Bae, E.; Kim, H.E.; Koh, E.; Kim, K.S.
    Phosphoglucomutase1 is necessary for sustained cell growth under repetitive glucose depletion (2014), FEBS Lett., 588, 3074-3080 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alpha-D-Glucose 1,6-bisphosphate dependent on Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 and Rosetta(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
T556A the mutant shows about wild type activity Homo sapiens
T556E the mutant shows about wild type activity Homo sapiens
T559A the mutant shows about wild type activity Homo sapiens
T559E the mutant shows about wild type activity Homo sapiens
T562A the mutant shows slightly reduced activity compared to the wild type enzyme Homo sapiens
T562E the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme with a C-terminus 6xHis tag completely loses activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate Homo sapiens
-
D-glucose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens P36871
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-
MCF-7 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
Homo sapiens D-glucose 6-phosphate
-
r

Synonyms

Synonyms Comment Organism
PGM1
-
Homo sapiens
phosphoglucomutase1
-
Homo sapiens

General Information

General Information Comment Organism
malfunction enzyme depletion is associated with declined cellular glycogen content and decreased rates of glycogenolysis and glycogenesis. Furthermore, enzyme depletion suppresses cell proliferation under long-term repetitive glucose depletion Homo sapiens
physiological function the enzyme is necessary for sustained cell growth under repetitive glucose depletion Homo sapiens